Introduction to enzymology 1400-125WENZ
There are following tasks of the lecture:
- presentation of different theories about enzymes catalytic power and their physical background
- description, based on examples, the relationship between enzyme catalytic properties and three-dimensional structure of enzymes protein
- description of a different enzyme kinetic in relation to complexity of enzyme structure and types of chemical reaction
- presentation of enzymes classes and different catalytic strategies e.g., evolution of enzyme complexes
- discussion on various types of enzyme specificity and contribution of amino-acid residues and cofactors to enzyme catalysis
- presentation of different types of bioenergetics systems, among others photosynthetic and mitochondrium chain
- demonstration of examples of enzyme utilization for biotechnology
In the laboratory part, students will learn the following topics:
1) Biochemical calculations and spectrophotometry
2) Determination of enzymatic activity and protein content by various analytical methods
3) Application of computer programs for simulation of enzymatic reactions
4) Experimental determination of kinetic parameters of enzymatic reactions
5) Purification of enzymes. Preparation of the purification table
6) Application of enzymes to measure concentrations of metabolites
Main fields of studies for MISMaP
biology
biotechnology
computer science
physics
Type of course
elective monographs
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Identifies and defines the tools of chemistry and physics necessary to understand the mechanisms of enzyme action at the molecular level.
Understands the structure and properties of basic types of biological macromolecules and the relationships between them, in particular understands the relationship between the spatial structure of enzyme proteins, the presence of functional residues, and catalytic properties of enzymes.
Knows the history of life on Earth and understands the reasons for the diversity of catalytic strategies.
Knows the basic laboratory techniques used in modern enzymology research.
Knows the appropriate statistical methods and computer techniques for analyzing data in enzymology.
Applies basic research techniques and tools used in enzymology research and knows how to explain the principles of their operation .
Reads scientific and popular science biological texts in a foreign language with understanding - Is able to analyze experimental work in the field of enzymology and bioenergetics.
Is able to analyze obtained experimental data and discuss them based on available literature.
Is able to use a computer and electronic sources to perform statistical analysis.
Is able to present obtained results in the form of a written paper or a multimedia presentation.
Learns independently the issues indicated by the mentor.
Expands interests within the natural sciences to include topics on mechanisms of catalysis, diversity of enzymes and enzyme systems.
Demonstrates the ability to work in a team.
Assessment criteria
Lecture:
The lecture exam is given in the form of a study of an experimental publication on enzymology. The student must analyze the content of the publication using the knowledge acquired during the lecture. The English-written publication is assigned randomly from a pool prepared by the instructor.
Laboratory:
Credit for the laboratory is given by:
1) attendance at at least 85% of the classes
2) completion of reports on the performed exercises
3) multimedia presentation on one of the performed exercises.
The final grade for the course is the arithmetic average of the grades from the laboratory and lecture.
Bibliography
Berg JM, Tymoczko JL, Stryer L Biochemia, PWN, Warszawa
Witwicki J, Ardelt W (red.) (1984) Elementy Enzymologii, PWN, Warszawa
Zgirski A, Gondko R (1998) Obliczenia biochemiczne, PWN, Warszawa
Materiały dostarczone przez prowadzącego
Notes
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Term 2025Z:
None |
Additional information
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