- Inter-faculty Studies in Bioinformatics and Systems Biology
- Bachelor's degree, first cycle programme, Computer Science
- Bachelor's degree, first cycle programme, Mathematics
- Master's degree, second cycle programme, Bioinformatics and Systems Biology
- Master's degree, second cycle programme, Computer Science
- Master's degree, second cycle programme, Mathematics
Plant transformation 1400-122TR-OG
Block of classes in which the lecture and practical classes will be performed
Lecture:
The aim of the lecture is to present current knowledge about the genetically modified plants. The lecture covers a number of general issues including generation of genetically modified plants (called transgenic plants) and their use in biotechnology. Lecture is understandable for people without well-established knowledge of genetics or plant biotechnology. The lecture addresses the following issues:
• methods used for obtaining genetically modiefied plants (transformation methods)
• characterization of DNA introduced into a plant recipient
• factors affecting transformation efficiency
• purpose of obtaining genetically modified plants
• methods for confirming the presence of an additional gene in a plant
Students during laboratory classes will:
a) get acquainted with one of the methods leading to the production of genetically modified plants (also called transgenic plants) of tobacco with the use of Agrobacterium tumefaciens (bacterial cultures, growth of plants on sterile solid media, regeneration of whole plants from single leaf cell)
b) isolate plant DNA
c) perform Polymerase chain reaction (PCR) to confirm that the obtained plants are genetically modified
d) discuss and interpret results of PCR
e) will carry out an experiment to show the trait that was acquired by plants used for transformation
Type of course
Mode
Course coordinators
Learning outcomes
KNOWLEDGE
- Has elementary knowledge of GMO plants
- Has knowledge of technics used in plant biotechnology
- Knows the basic techniques and tools in research related to the preparation and analysis of genetically modified plants, understands the importance of experimental work in plant biotechnology and is able to describe the importance of molecular analysis in biological research
- Knows the basic vocabulary of genetically modified plants
ABILITIES:
- Uses basic techniques appropriate for plant biotechnology
COMPETENCES:
- Understands the need to expand his knowledge
- Understands the need to provide information on genetically modified plants to the public and is able to convey this information in an understandable way
Assessment criteria
Assessment methods and assessment criteria
The final grade is the grade from the exam. The exam will be written (a descriptive answer to the questions). Students can take notes with them.The condition of admission to the exam is appropriate class attendance (permissible absence from one class) and submission of the report.
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
- Inter-faculty Studies in Bioinformatics and Systems Biology
- Bachelor's degree, first cycle programme, Computer Science
- Bachelor's degree, first cycle programme, Mathematics
- Master's degree, second cycle programme, Bioinformatics and Systems Biology
- Master's degree, second cycle programme, Computer Science
- Master's degree, second cycle programme, Mathematics
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: