Analysis of Biologically Active Compounds B - laboratory 1200-1CHMAZBL5
The laboratory covers the basics of analysis of organic compounds, including bioactive compounds. Students perform all the exercises practically and individually.
Students learn and practically apply the basic techniques of analysis, identification and determination of the structure of organic compounds.
That includes:
- spectroscopic methods, in particular proton nuclear magnetic resonance (1H NMR), UV-Vis and IR spectroscopy
- basic chromatographic techniques - Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC)
- techniques of separation and isolation of organic compounds: silica gel column chromatography, extraction, distillation, crystallization
- classical methods of chemical analysis
The laboratory has an extended program compared to the "A" version.
In addition, students will refere their own results to available literature data (in particular, spectral databases), thus practicing the ability to interpret the results and also practicing common ways recording data in accordance with accepted standards.
The laboratory also strengthens the previously adopted standards of work in accordance with health and safety regulations, as well as the ability to conduct observations and laboratory notes.
Particular procedures of synthesis and instructions for exercise are provided, in advance, by the instructors.
Estimated student workload per semester: 100 hours, including:
- 60 hours participation in laboratories
- 28 hours preparations to tests
- 12 hours preparation of reports
Type of course
Mode
Requirements
Prerequisites (description)
Course coordinators
Learning outcomes
Knowledge. Upon completion of the programme, the graduate:
- (K_W12) demonstrates advanced knowledge of methods and techniques of synthesis and identification of organic compounds, with particular emphasis on pharmacologically significant and biologically active substances, using chemical, chromatographic, spectroscopic, and diffraction methods;
- (K_W14) possesses in-depth theoretical knowledge of various types of molecular spectroscopy and its applications, in particular for the study of biological systems;
- (K_W18) has advanced understanding of the construction, principles of operation, and application of modern measurement instrumentation supporting research in chemistry, biochemistry, and molecular biology;
- (K_W19) understands occupational health and safety principles at a level sufficient to ensure responsible and safe work in chemical, biochemical, and molecular biology laboratories.
Skills. Upon completion of the programme, the graduate:
- (K_U10) is able to identify selected organic and bioorganic compounds using chemical, biochemical, molecular biology, chromatographic, and spectroscopic techniques;
- (K_U12) is able to apply spectroscopic and biospectroscopic methods for the analysis of molecular structure and properties in both gaseous and liquid phases;
- (K_U15) is capable of planning, conducting, and interpreting fundamental research, experiments, observations, and computer simulations in chemistry, biochemistry, and molecular biology, and of critically evaluating outcomes, including the identification and discussion of potential measurement errors;
- (K_U16) is able to design, configure, and operate selected measurement systems, perform determinations of physicochemical parameters, and assess the validity and reliability of experimental data;
- (K_U21) is able to organise individual and collaborative work within research projects, critically evaluate progress, independently undertake and initiate basic research tasks, and effectively contribute to teamwork.
Assessment criteria
Attendance is mandatory. Excused absences from two classes are permitted, which must be made up on a different date. One additional class date is planned.
- Requirements for passing the course:
1) Completion of all individual excerices and approval of written reports of each excercise
2) Passing all the partial test. Each test consists of two parts - theoretical (8 points) and concerning the performance of a given exercise (2 points). It is required to obtain at least 50% from all partial tests and at least 50% from the theoretical part of each test (4 points). The theoretical parts of each test can be corrected during the semester during classes upon appointment. Students propose when they want to retake tests. The part of the test concerning the performance of a given exercise cannot be corrected.
- The final grade consists of:
1) grades obtained from tests,
2) grades obtained from exercises,
3) assessment of the overall quality of work, in particular compliance with the requirements of occupational health and safety.
- Conditions for passing in the correction session:
Students who did not complete all practical exercises within the deadline may perform ONE exercise during the correction session. Failure to perform more than one exercise makes it impossible to pass a course.
Students who did not meet the credit criteria due to tests criteria can correct any number of tests in a maximum of two appointments.
Schedules for retaking the tests and practical exercises are set individually.
Practical placement
Not applicable
Bibliography
(all literature is given in Polish, for english version please contact the teacher)
1. K. Bańkowski, A. Krawczyk, R. Siciński, J. Stępiński, A. Temeriusz – Ćwiczenia z organicznej analizy jakościowej i chemii bioorganicznej - Wyd. UW, 1990.
2. A. Vogel - Preparatyka organiczna - WNT, 1984
3. Z. Witkiewicz - Podstawy chromatografii i technik elektromigracyjnych - PWN, 2017
4. Z. Witkiewicz – Podstawy chromatografii - WNT, 2000
5. M. Szafran, Z. Dega-Szafran - Określanie struktury związków organicznych metodami spektroskopowymi - PWN, 1988
6. R. M. Silverstein, F.X. Webster, D.J. Kiemle Spektroskopowe metody identyfikacji związków organicznych - PWN 2007
7. W. Zieliński, A. Rajca (red) - Metody spektroskopowe i ich zastosowanie do identyfikacji związków organicznych - WNT, 1995
8. C.N.R. Rao, – Spektroskopia elektronowa związków organicznych - PWNT, 1982
9. J. McMurry - Chemia organiczna, części 1-5 - PWN, 2007 (lub późniejsze): rozdziały dotyczące określania struktury związków
10. J. Clayden, N. Greeves, S. Warren, P. Wothers - Chemia organiczna - WNT, 2009 (lub późniejsze): rozdziały dotyczące określania struktury związków
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: