Experimental embryology of plants 1400-226EMBR
Lectures: flowering and its molecular regulation: flower structure, transition from vegetative to generative phase, inflorescent and flower meristem, induction of flowering, MADS box transcription factors and their role in flowering process, structure and development of ovule, developmental stages of female gametophyte, structure and development of anther and developmental stages of male gametophyte, pollination and fertilization processes, development of embryo and endosperm, formation and development of seeds and fruits, germination process, epigenetic reprogramming and the role of imprinting process in the development of male and female gamethophyte.
Practicals: study of flower and inflorescent structure of selected species, isolation of ovules and of embryo sacs, identification of microsporogenesis and microgametogenesis stages, pollen grains germination in vitro culture, detailed analysis of cytological aspects of different stages of pollen tube growth and development, isolation of embryos, somatic embryos culture, fluorescent method of detection of selected proteins of embryo sac with the use of GFP protein, analysis of defects in Arabidopsis flower development and of ovule development.
Type of course
Mode
Prerequisites (description)
Learning outcomes
KNOWLEDGE
1. Understanding complexity of developmental processes, the need for interdisciplinary approach, based on empirical data to find their solutions.
2. Knowing structural, genetic, metabolic and functional diversity of developing and differentiating plant organisms.
3. Understanding rules and molecular, cellular, physiological mechanisms of development and function of organisms.
4. Knowing various techniques and advanced laboratory tools used in experimental embryology of plants and planning their application in proper and efficient experiments.
SKILLS
1. Applying efficiently research and popular texts in Polish and English on developmental plant biology. Ability to communicate in English at the B2+ level.
2. Possessing skills to find in appropriate literature possible reasons for failed experiments and modify them.
3. Possessing ability of preparing presentations in Polish and English at the B2+.
4. Ability for team work on advanced projects on experimental embryology of plants.
SOCIAL AWARENESS
1. Ability to popularize advanced of modern developmental plants biology and experimental embryology of plants.
2. Ability to improve joint work of the team.
3. Understanding the need for continuous learning.
4. Carefully and critically interpreting knowledge and information coming from scientific literature and mass media.
Assessment criteria
Written examination or final project (to be arranged with the students)
Practical placement
does not apply
Bibliography
Wojtaszek i wsp.. Biologia komórki roślinnej, tom I i II, PWN 2007
E-C Pua, M.R. Davey (eds) Plant Developmental Biology-Biotechnological perspetives, vol.1 i 2 Springer-verlag, Berlin, Heidelberg, 2010
Additional information
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