Environmnetal analysis 1400-ZS-ANS-c

On the lecture the following issues will be raised: fundamentals of analytical chemistry and instrumental analysis. Basic knowledge of analytical and bioanalytical analysis (sample, quality, good laboratory practice, standard). Specific conditions required in the environmental laboratory. Sources of errors in trace analysis. On-field sampling of soil, plants and water. Basic information about mineralization accelerated solvent extraction, drying, sieving, liofilization, total content determination, filtration, acidification and dilution. Selection of the proper analytical method according to the sample type and expected concentration of analyte. Methods of sample stabilization. Certified reference materials. Application of chromatography, electrochemistry and spectrometry in the environmental analysis. Validation of analytical results and their comparison with respected norms. Retrospective studies in the environmental monitoring. Analytical aspects of wastes utilization (extractability test, save deposition of wastes, stability of wastes) and wastes management. An integral part of the subject is a laboratory in the field of instrumental analysis and preparation of laboratory samples for analysis. The student performs exercises in the field of decomposition of solid samples (mineralization in a microwave oven), filtration of suspension from water, determination of selected analytes by spectrometric methods (e.g. UV Vis) and electrochemical methods (biosensors) and methods of separation of analytes (solid phase extraction, liquid and gas chromatography ). Time-consuming: Exercises 7 x 4 hours = 28 hours of work with the teacher and 12 hours of own work (learning and writing a report) and 30 hours of lecture to be listened to and 3 hours of discussion with teachers during the obligatory meeting.