Proteomics 1400-225PROT-en
In this course students get acquainted with the proteomic methodology. In practical part students will purify protein complexes by Tandem Affinity Purification method and analyze there composition using Mass Spectrometry. In theoretical part students will gain knowledge about various applications of Mass Spectrometry in biology and medicine. This includes the application of mass spectrometry for the identification of proteins and their posttranslational modifications, the information on typical protocols of sample fractionation and preparation, types of mass spectrometric experiments used (including data dependent MS/MS experiment as a basis of modern proteomic analysis) and software tools for the data analysis (database search) and verification steps. Lecture is illustrated with the examples of application of proteomic methodology for solving various problems of bio-medical sciences.
Type of course
Mode
Course coordinators
Assessment criteria
The final assessment is based on following components:
1. assessment of activity in class.
2. mid-semester presentation on the proteins purified and progress of the experiment.
3. final report and presentation at the end of class.
Bibliography
Gary Siuzdak (1996) Mass Spectrometry for Biotechnology, Academic Press (ISBN 0-12-647471-0) - brak polskiej pozycji dot. proteomiki.
E. de Hoffmann, J. Chatette, V. Stroobant (1994) Spectrométrie de masse; Masson Editeur, Paris; tłum. L. Konopski (1998) Spektrometria Mas; Wydawnictwa Naukowo-Techniczne (ISBN 83-204-2291-4).
Robert A. W. Johnstone, Malcolm E. Rosse (1996) Mass spectrometry for chemists and biochemists; Press Syndicate of the University of Cambridge; tłum. pod red. Krystyny Jurkowskiej (2001) Spektrometria mas; Wydawnictwo Naukowe PWN SA (ISBN 83-01-13605-7).
Notes
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Term 2023Z:
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Term 2024Z:
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Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
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