Bioprocessing Engineering 1400-113IBP

The lecture focuses on principles of industrial processes involving microorganisms, enzymes, plant and anima cells. The bioreactor engineering, down- and up-stream processing are the main subject presented.
1.Bioreactor engineering principles 16 h
1.1.Balances of microbial growth 4 h
Elemental balances, degree of reduction, energetic of growth, maintenance, structural balances.
1.2.Enzymatic kinetics 2 h
Michaelis - Menten kinetics. Estimation of .Michalis-Menten model coefficients. Mechanisms of inhibition.. Effects of temperature and pH.. Complex enzymatic reactions
1.3.Microbial growth models 1 h
Model of microbial kinetics. Non-structured and structured models. Segregated models.
1.4.Reactor engineering principles. 1 h
Ideal and non-ideal reactors. Macro and micro-mixing.
1.5.Submerged cultures. 2 h
Batch culture. Fed-batch cultures. Continuous cultures.
1.6.Solid state fermentation. 1 h
1.7.Enzymatic reactors. 1 h
1.8.Bioreactors with immobilized enzymes and cells. 1 h
1.9.Membrane bioreactors. 1 h
1.10.Design of bioreactors. 2 h
Aeration and mixing systems. Heat transfer in bioreactors. Scale-up of bioreactors.
2.Up-stream processing 3 h
2.1.Optimizing of medium compositions 1 h
2.2.Sterilization. 1 h
Sterilization techniques. Thermal sterilization. Absolute filtration.
2.3.Disruption and mixing of solid materials. 1 h
3.Down-stream processing. 11 h
3.1.Biomass separation 1 h
Filtration. Centrifugation. Sedimentation
3.2.Cell disruption.. 1 h
3.3.Membrane methods of separation. 2 h
Dialysis. Elektrodialysis.. Ultrafiltration. Reverse osmosis.
3.4.Electrokinetic and chromatography methods. 1 h
3.5.Precipitation. 1 h
3.6.Extraction 1 h
3.8.Distillation and rectification 2 h.
3.9.Drying of biological materials. 1 h
3.10.Selection of separation methods. 1 h