Methods of Organic Compounds Identification Laboratory B 1200-1MIZOBL5

The goal of the course is to learn the ability to:
- use chemical and physical methods of identification of simple organic compounds,
- perform chemical reactions identifying functional groups - determining physical properties of a compound,
- apply chromatography to identify simple organic compounds; choose thin-layer chromatography conditions,
- apply chemical methods to separate components of simple organic compounds, isolate biological material components and obtain active components from pharmaceuticals,
- apply column chromatography to separate components of mixtures of natural compounds (choosing conditions, adsorbents and eluents),
- apply UV-Vis spectroscopy to identify biological material components,
- compare experimental data with literature,
- analyze spectral data, identify compounds basing on infrared (IR) spectra and 1H NMR, 13C NMR nuclear magnetic resonance spectra.
Excercise list:
Exercise 1. Reactions identifying functional groups.
Exercise 2. Identification of unknown organic compound.
Exercise 3. Separate and identification of components of mixture of two organic compounds.
Exercise 4. Application of thin-layer chromatography to identify components of mixture of two solid organic compounds.
Exercise 5. Isolation and separation of biological material components.
Exercise 6. Identification of biological material components by means of UV-Vis spectroscopy; comparing experimental data with literature.
Exercise 7. Analysis of drugs by chemical and chromatographic methods.
Exercise 8. Identification of mixture components from exercise 3. and 4. by spectroscopic methods (IR, 1H NMR, 13C NMR).

Type of course

obligatory courses

Prerequisites (description)

Knowledge of organic chemistry basics. Furthermore, student should be able to conduct synthesis of simple organic compounds and be proficient with laboratory techniques used in organic chemistry: crystallization, extraction, simple distillation, vacuum distillation, steam distillation. Student should know the laboratory safety rules.

Learning outcomes

Upon completing the laboratory student:
- knows theoretical basis for experimental methods utilized in identification of mixture components and determining structures of simple organic compounds,
- knows experimental methods utilized in separation of mixtures of organic compounds,
- knows experimental methods utilized in identification of organic compounds,
- knows applications of organic compounds identification methods in biology and medicine,
- is able to pick separation method and separate a mixture of organic compound by chemical and chromatographic methods,
- chooses conditions for thin-layer and column chromatography,
- is able to use ultraviolet-visible (UV-Vis) spectroscopy method and record and analyze UV-Vis spectra,
- recognizes functional groups basing on infrared spectrum, analyzes IR spectra,
- is able to identify simple organic compounds based on their NMR data, analyzes 1H NMR and 13C NMR spectra,
- is able to reliably analyze experiment results.

Assessment criteria

Mandatory attendance
Tests verifying preparation for class – 55%
Exercise execution, written description of exercise – 30%
Cooperation in collective exercises,
activity, self-reliance, other – 15%

Practical placement

not applicable

Bibliography

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b) J. McMurry - Chemia Organiczna - PWN, 2005.
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