Molecular biology laboratory 1100-2BB21
The aim of the laboratory is to acknowledge the students with chosen techniques of molecular biology.
1. Chosen methods of nucleic acids isolation and analysis.
2. Restriction enzymes - as an instrument in genetic engineering.
3. Designing of constructs based on plasmid vectors.
4. Amplification of a DNA fragment using polymerase chain reaction (PCR).
5. Cloning of a DNA fragment in E. coli on a plasmid vector.
6. Obtaining recombinant protein using a method of overexpression in bacterial cells.
7. Assessment of purity and concentration of the protein.
Student's amount of work:
The laboratory = 90 hours
Preparation of the reports = 45 hours
Unassisted preparation for the exercise = 45 hours
Preparation of a chosen scientific article = 30 hours
Total 210 hours
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Having learned the material covered by the classes, the student:
KNOWLEDGE
1. knows chosen methods of isolation of nucleic acids from bacterial cells, (K_W04, K_W06, K_W07)
2. knows chosen methods of analysis of nucleic acids, (K_W04, K_W06, K_W07)
3. knows a method of DNA fragment amplification using the PCR technique, (K_W04, K_W06, K_W07)
4. knows chosen methods of assessment of protein purity and concentration (K_W04, K_W06, K_W07)
5. knows chosen heterologic protein expression systems in E. coli cells. (K_W04, K_W06, K_W07)
ABILITIES
1. can isolate plasmid DNA using alkaline lysis, (K_U01, K_U04)
2. can transform bacterial cells with a plasmid vector - using a heat-shock method or via electroporation, (K_U01, K_U04)
3. can apply the PCR technique for DNA fragment amplification, (K_U01, K_U04)
4. can clone DNA fragments in E. coli on a plasmid vector,(K_U01, K_U04)
5. can obtain recombinant protein using overexpression in bacterial cells, (K_U01, K_U04)
6. can determinate protein concentration in a solution, (K_U01, K_U04)
7. can assess the purity of the obtained sample, (K_U01, K_U04)
8. can interpret the results of the performed experiments. (K_U04, K_U09)
ATTITUDES:
1. is aware of the meaning of research ethics and responsibility for reliability of the obtained results, (K_K04, K_K05)
2. understands the possibilities of using techniques of genetic engineering in biotechnology, medicine, diagnostics, criminalistics and in the industry. (K_K06, K_K07)
Assessment criteria
Submitting of the cards of every class
Bibliography
1. P. C. Turner, A.G. McLennan, A.D. Bates, M.R.H. White, „Biologia Molekularna" z serii "Krótkie Wykłady" - PWN, Warszawa -wybrane zagadnienia
2. T.A Brown „Genomy” PWN, Warszawa – wybrane zagadnienia
3. R. Słomki (red.) „Analiza DNA” Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu, Poznań 2008
4.T.A Brown „Gene Cloning & DNA Analysis" Blackwell Publishing -
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: