Molecular biology with genetics, part II 1100-2BB20
The course plan:
1. Objects of genetic studies - model organisms.
2. Elements of bacterial genetics: mobile genetic elements of bacteria (plasmids, insertion sequences, transposones) and mechanisms of horizontal gene transfer.
3. Instruments of genetic engineering - enzymes used for DNA and RNA manipulation and modification. Mechanisms of restriction and modification in bacteria - discovery of restriction enzymes.
4. Polymerase chain reaction (PCR) - ingredients and course of PCR, types of PCR (i.a. localised PCR, multiplex PCR, RT-PCR, Real-Time PCR), applications.
6. DNA cloning:
a/ bacterial plasmid vectors, stages of PCR product cloning on a plasmid vector in E. coli,
b/ bacteriophage-derived vectors,
c/ yeast vectors, artificial yeast chromosome,
d/ vectors used for higher eukaryotes, methods of animal cell transfection
e/ gene cloning in plants.
7. Methods of DNA sequencing, sequencing of genomes.
8. Methods using hybridisation of nucleic acids.
a/ labelling of nucleic acids
b/ hybridisation of DNA with molecular probes - Southern method,
c/ identification of RNA molecules - northern hybridisation,
d/ microarrays.
9. Production of proteins from the cloned genes,
a/ expression systems,
b/ methods of protein purification
c/ immunotechniques of protein studies.
10. Human genetics:
a/ elements of cytogenetics: chromosome classification, principles of notation, determination and analysis of karyotype, in situ hybridisation,
b/ chromosome aberrations,
c/ studies on DNA polymorphism,
d/ genetic diseases: inheritance, diagnostics,
e/ gene therapy.
Student's amount of work:
The course = 30 hours
Preparation for the exam = 45 hours
Total = 75 hours
Mode
Prerequisites (description)
Learning outcomes
Having learned the material covered by the course, the student:
KNOWLEDGE
- knows elements of bacterial genetics: plasmids, horizontal gene transfer, (K_W01)
- knows basic techniques of genetic engineering and their application in molecular diagnostics and in criminalistics. (K_W01, KW04, K_W06)
ABILITIES:
- can present and explain basic techniques of molecular biology used in medical diagnostics and criminalistics. (K_U02)
ATTITUDES:
- understands the possibilities of using techniques of genetic engineering in biotechnology, diagnostics of inherited diseases and in criminalistics. (K_K05, K_K06)
Assessment criteria
Final written exam, comprising of questions with a choice of answers or a short answer and tasks based on pictures
Bibliography
1. P. C. Turner, A.G. McLennan, A.D. Bates, M.R.H. White, „Biologia Molekularna" z serii "Krótkie Wykłady" - PWN, Warszawa - wybrane zagadnienia
2. T.A Brown „Genomy” PWN, Warszawa – wybrane zagadnienia
3. T. Strachan, A. P. Read „Human Molecular Genetics 2” Wiley-Liss
4. R. Słomki (red.) „Analiza DNA” Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu, Poznań 2008
5. J. Bal (red.) „Biologia molekularna w medycynie”, PWN, Warszawa, 2006
6. J. Baj i Z. Markiewicz (red.) „Biologia Molekularna Bakterii”, PWN, Warszawa, 2006
6. I. Kątnik-Prastowska (red.) „Immunochemia w biologii medycznej” PWN, Warszawa, 2009
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: