Animal embryos and embryonic stem cells 1400-226ZZKMZ
The subject is directed to students who are interested in animal embryology and the possible application of embryonic cells and in vitro cultured embryonic stem cells in biological studies. The course consists of practical classes, which will familiarize students with experimental models such as mouse oocytes and embryos (control of cell divisions during meiosis, fertilization, regulation of gene expression in the embryo, regulative capacities of the mouse embryo and derivation of primary cultures of embryonic and foetal cells). Each practical class will be preceded by a short theoretical introductory lecture illustrating the background of the performed experiment. Subsequently, students will be working individually following the instructions and guidelines of the instructors.
The following topics are covered during the course:
1. Regulation of meiosis in the mouse oocytes.
2. Fertilization of mouse oocytes
3. Epigenetic diversity of pronuclei in the mouse zygote
4. Transition from maternal to embryonic control of gene expression using transgenic mice expressing various fluorescent proteins.
5. Formation of the primary cell lineages in the mouse embryo.
6. Derivation, in vitro culture and application of mouse embryonic stem cells (ES cells) and establishment of mouse primary embryonic fibroblasts.
7. Regulative capacities of the early mouse embryo – the creation of aggregation chimaeras.
NOTE: The course will consist of 6 classes chosen from the 7 proposals listed above.
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Understands the rules as well as the molecular, cellular and physiological mechanisms of the development and functioning of organisms(K_W05).
Knows various research techniques and tools used in embryology and cell culture (e.g. isolation of oocytes and embryos, creating chimaeras, indirect immunofluorescence, setting up cell cultures) and properly plans their use to solve the tasks (K_W10).
Knows the principles of research planning and conducting experiments with the use of specialized methods used in embryological research (K_W13).
Uses scientific and popular science biological texts in Polish and English and communicates in English at the B2+ level (K_U03).
Can critically develop a selected scientific problem based on literature data and the results of his research, formulating his own opinions and conclusions (K_U08).
Has the ability to prepare and deliver oral presentations on a topic in the field of embryology in Polish and English at the B2+ level (K_U11).
Applies the principles of research ethics, resolving dilemmas related to the use of animals in research (K_K06).
Assessment criteria
The final evaluation course will be based on:
• presence (at least 5 out of 6 two-day thematic blocks),
• student’s work and engagement during the classes,
• presentation prepared by two students and related to the subject of the course (the form of presentation and ability to interpret and discuss the results in context of the current knowledge will be taken into account).
• knowledge of the issues presented by instructors during the introductory lectures.
Practical placement
Not applicable
Bibliography
"Ćwiczenia z biologii rozwoju zwierząt" pod red. M. Maleszewskiego, Wydawnictwa Uniwersytetu Warszawskiego (2007)
„Developmental Biology” S.F. Gilbert, wyd. 6-9. Sinauer Associates, Inc., Sunderland, Massachusetts USA
„Hodowla komórek i tkanek”; pod red. S. Stokłosowej; PWN (2006)
„Biotechnologia Zwierząt”; pod red. L. Zwierzchowskiego, K. Jaszczaka, J.A. Modlińskiego; PWN (1997)
„Genetyka molekularna”; pod red. P. Węgleńskiego, PWN (2008).
„Zarodkowe komórki macierzyste – w poszukiwaniu pluripotencji”. M.A. Ciemerych; Postępy Biologii Komórki 35, 203 (2008).
Notes
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Term 2023Z:
None |
Term 2024Z:
None |
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: