Monitoring of genetically modified organisms 1400-216MOGZ

LECTURE
Definition of genetically modified organisms and the methods used to produce GMOs; the Cartagena Protocol on Biosafety to the
Convention on Biological Diversity, Directive 2001/18/EC of the European Parliament and of the Council and their references to the Convention on Biological Diversity; GM bacteria monitoring objectives; The objectives of the monitoring of GM crops; Monitoring as a tool of environmental risk assessment; Case Specific Monitoring - short-and long-term monitoring the effects of the release into the environment of selected genetically modified plants and bacteria, adjusting the spatial scale for the purpose of monitoring, assessment of environmental risks; Monitoring of GMOs in processed foods, fresh, crops in the fields; Detection techniques of GM microorganism based on DNA analysis; The most commonly used vectors, marker genes, genes modifying the characteristics of microorganisms, GM microorganisms and horizontal gene transfer; Methods for determining the number of GM microorganisms (enzymatic, fluorescent, PCR, MPN-PCR, real-time PCR); General Surveillance - monitoring as a tool to control harmful for human health effects of the use of GM crops; Monitoring the presence of GM plants, parts of their bodies and products in the environment; Range of monitoring of GM crops: all organisms in the environment (crops and wild type after crossing from GMOs), parts of the body (including pollen, fragments of bodies) in the air, water, soil, sewage sludge, food fresh and processed in the gastrointestinal tract of animals); Identifying ways of spread GM crops in the environment; The rules for creating a plan for monitoring progress (including the response to the demand, the choice of indicators, methods, places, method of preparation of the report); The most commonly used vectors, marker genes, genes modifying the characteristics of plants; The most common species of plants and modified features; Methods of monitoring, taking into account the duration and site selection (including types of methods, sensitivity, adaptation to needs), standardization of methods; Principles registration of GM crops.
LABORATORY
Students will become familiar with the following methods used in the analysis of the presence of GMOs (bacteria and plants):
Based on the analysis of DNA (qualitative and quantitative analysis): Design of primers for PCR for detection of sequences most frequently introduced into plants, qualitative and quantitative analysis of DNA,