Basics of in vitro cell culture 1400-116PHKinv
Lecture:
1. Why do we culture plant cells and tissues
2. Totipotentiality of plant cells and organogenesis
3. Types of plant cell and tissue culture in vitro
4. Somatic embryogenesis and somaclonal variability
5. Production of secondary metabolites in bioreactors
6. Biofarming
7. The animal cell and tissue culture laboratory equipment
8. The conditions, medium and methods of animal cell cultures
9. The three dimensional cell and tissue cultures
10. Obtaining of primary cell cultures and cell lines
11. Development of animal cell culture techniques
12. Semi-technique scale cultures
13. Application of cell and tissue cultures in science, biotechnology and medicine
Laboratory:
Artificial seeds
Micro-reproduction
Development of orchid somatic embryos.
Evaluation of the growth rate of BY2 tobacco cell suspension cultured under various conditions.
Explant orientation and the ability to induce callus from the sunflower hypocotyl.
Organization of laboratory, materials, conditions of cell culture, sterilization
Culture of adherent and not adherent cells
Freezing procedure and recovery of cells from liquid nitrogen
Measurement of cells growth in culture
Measurement of survival rate
Basic cytochemical staining
Obtaining of primary cell culture
Differentiation of cells in culture in vitro
Main fields of studies for MISMaP
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Having completed the course and the lab student:
Knowledge
1. Has elementary knowledge in selected basic areas of cell culture in biotechnology and understands the relationships and dependencies between different disciplines of life science.
2. Has knowledge about the cell culture basics in life sciences, conceptual categories and terminology related to cell culture and knowledge about the research methods, and also is able to point out the most important scientific discoveries in the history of biotechnology, including in vitro cultures.
3. Has knowledge about basic cell culture techniques and tools in the study of life science and understands the importance of experimental work in biotechnology and is able to describe the importance of molecular analyzes in biological and medical research.
4. Has knowledge about the technological aspects of cell culture in biotechnology.
Skills
1. Applies basic techniques, appropriate for biotechnology, including in vitro cultures.
2. Shows the ability to use available sources of information, including electronic sources.
3. Carries out simple research tasks under the supervision of a tutor.
4. Shows the ability to acquire and characterize biological material.
Social competence
1. Student understands the biological phenomena and processes in nature.
2. Student is responsible for his own work and equipment; respects self and others work.
3. Student shows the ability to work effectively in a team.
4. Understands the need to provide information about new developments related to cell culture in biotechnology and can provide this information in an understandable way.
Assessment criteria
The acceptance of the regulations is required.
Work in a lab coat.
Two absences are allowed.
People who are more than 15 minutes late will not be able to participate in the classes.
Laboratories
The laboratory is passed if: the student was present and worked satisfactorily, i.e. performed all the required tasks. The practical part of the laboratory must be passed.
Passing the laboratory - oral test (animal cell culture) and presentation ( plant cell culture). The final laboratory grade is the mean of both parts. The pass criterion is to show that the knowledge has been mastered.
Lecture - written exam - open and test questions. Only students who passed the laboratory can take the exam. The pass criterion is to show that the knowledge has been mastered.
Practical placement
no
Bibliography
Podstawy biotechnologii (red. Colin Ratledge, Bjorn Kristiansen, PWN 2011)
Biotechnologia roślin (red. Stefana Malepszy, Warszawa, PWN 2001),
Biotechnologia molekularna (Jerzy Buchowicz, Warszawa PWN 2006),
Komórki roślinne w warunkach stresu. Tom II (red. Adam Woźny i Krystyna Przybył, Wydawnictwo Naukowe UAM, Poznań 2004).
Plant Tissue Culture Techniques and Experiments (Roberta H. Smith, Academic Press, ISBN #0-12-650342-7).
Hodowla komórek i tkanek; Stanisława Stokłosowa; PWN
CULTURE OF ANIMAL CELLS A MANUAL OF BASIC TECHNIQUE AND SPECIALIZED APPLICATIONS R. Ian Freshney
Cell culture for biochemists; R.L.P. Adams; Elsevier
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: