Liquid Chromatography mass spectrometry 1200-SZD-LCMS
The following problems are discussed during the lecture:
1. Fundamentals of High Performance Liquid Chromatography
2. Fundamentals of mass spectrometry
3. Analysers in Mass spectrometry (quadrupole, ion trap, time of flight etc)
4. Ionization modes in MS
5. Tandem mass spectrometry
7. Scans modes in MS (single ion monitoring, product ion scan, single reaction monitoring, neutral loss precursor ion scan etc)
8. Interpretation of mass spectrum
For each lecture, there is homework consisting of a set of tasks, a test, an animation, and a crossword puzzle. PhD students must complete it before the next lecture
Type of course
Course coordinators
Learning outcomes
Student knows and understands the principles of LC/MS and how to use it in practical aspects. Student knows how to interprate the simple mass spectrum. Student knows how to choose the method of ionization and how to prepare samples for LC/MS analysis. He speaks English to a degree enabling participation in an international scientific and professional environment, in particular writing articles for international journals
Assessment criteria
Performing all housework. Positive evaluation of the final essay
Bibliography
1.R.A.W. Johnstone, M.E. Rose, Mass spectrometry for chemists and biochemists, Cambridge University Press 1996
2. E. de Hoffmann, J. Charette, V. Stroobant. Mass spectrometry principles and application, John Wiley & Sons, Ltd 2002.
3. R. E. Ardrey, Liquid Chromatography mass spectrometry, John Wiley & Sons, Ltd 2003
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: