Application of Scanning Probe Microscopy to Biological Samples 1200-3MON29L
Fundamentals of scanning tunneling microscopy (STM) and electrochemical scanning tunneling microscopy (EC-STM). Application of STM and EC-STM techniques for structural studies of biomimetic membranes including lipid films. Application of STM for molecular recognition imaging, identification of functional groups and metallic centers within the proteins, electron transfer through organic molecules.
Fundamentals of atomic force microscopy (AFM). Different AFM modes: Contact (C-AFM), Non-contact/Intermittent contact (NC/IC-AFM), Conductive Probe (CP-AFM), Lateral Force (LFM), Electrostatic Force (EFM), Chemical Force (CFM), Force Spectroscopy. Imaging and probing of biological samples, biomimetic membranes and cells. Measurements of adhesion forces on model membranes. Chemical specificity in AFM-based imaging. Modification of AFM probes with (bio)molecules for molecular recognition imaging and the identification of specific membrane regions.
Type of course
elective courses
Mode
Course coordinators
Learning outcomes
After completing the course, the student should demonstrate the knowledge of the fundamentals of STM and AFM techniques as well as understanding the research methodology related to imaging of biological and biomimetic samples. The acquired knowledge should enable students to correctly identify and select appropriate experimental tools to solve specific research problems and to carry out the physicochemical characteristics of the analyzed systems. One of the assumed effects is the acquisition of the ability to use knowledge from various fields of science (chemistry, physics, biology) for effective research planning - from research hypotheses through experiments to proper critical analysis of results.
Assessment criteria
Assessment in the form of a quiz. The pass mark is to obtain a minimum of 51% of the maximum number of points in the quiz. The permissible number of excusable absences is 2.
Practical placement
not applicable
Bibliography
1. "Scanning Probe Microscopies Beyond Imaging Manipulation of Molecules and Nanostructures" ed. Paolo Samori, Wiley VCH, Weinheim 2006.
2. "A practical Guide to Scanning Probe. Microscopy.", R. Howland, L. Benatar. Park Scientific Instruments, 1993-1996
3. P.W. Atkins "Physical Chemistry" Oxford Univeristy Press, 2006.
4. 4. "Review of Progress in Atomic Force Microscopy" S. Maghsoudy-Louyeh, M. Kropf, B. R. Tittmann, 2018, DOI: 10.2174/1874440001812010086.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: