Trace analysis of the organic pollutants in the environment using capillary gas chromatography 1200-2SPEC392M
1. Capillary gas chromatography: separation mechanism, interaction in solution, stationary phases, retention index, signal separation, capillary columns, temperature programming, detectors, qualitative and quantitative analysis.
2. Gas chromatography-mass spectrometry: mass spectrometry basics, ionization methods, high resolution and quadrupole spectrometers, qualitative analysis using GC/MS, single ion monitoring (SIM), tandem mass spectrometry (MS/MS), liquid chromatography-mass spectrometry (LC/MS), solving a structure using mass spectrometry.
3. Sampling and sample preparation prior to chromatographic analysis: (i) air: whole air sampling, adsorptive sample enrichment, cryogenic sampling, analytes recovery; (ii) water: sampling, liquid-liquid extraction, solid phase extraction (SPE and SPME), head space analysis; (iii) soil: preparation of soil samples, Soxhlet extraction,, sample clean up and enrichment. Uncertainty in environmental analysis
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
After the completion of this course students will understand the major problems of the trace analysis of organic pollutants in the environment using the capillary gas chromatography (GC). They will also learn about the sample preparation techniques and methods required for GC analysis.
Assessment criteria
test
Practical placement
NA
Bibliography
1. Z. Witkiewicz, W. Wardencki Chromatografia gazowa. teoria i praktyka PWN, Warszawa 2018.
2. R. A. W. Johnstone, E. R. Malcolm, Spektrometria mas. Podręcznik dla chemików i biochemików,(Mass spectrometry for chemists and biochemists), PWN, Warszawa, 2001.
3. J. Namieśnik, Z. Jamrógiewicz, M. Pilarczyk, L. Torres, Przygotowanie próbek środowiskowych do analiz (Preparation of environmental samples for analysis), WNT, 1999.
Additional information
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