(in Polish) Bioanalityka próbek naturalnych 1200-2BLOK3-AW3
Tasks to be realised include: defining the research objective and planning its implementation; collecting biological and environmental samples to ensure sample stability; methods for sample preservation to maintain primary stability; identifying sources of errors in sample analysis while maintaining representativeness; relationship between the form of element occurrence and its toxicity; sources of errors in speciation analysis; analytical methods used for determining different analyte forms; methods of analyte extraction from sample matrices (mineralisation, solvent and assisted extractions, solid-phase extractions); definition and construction of sensors/biosensors, with special emphasis on sensors with electrochemical detection; methods receptor layers formation and effective immobilisation of the bio-receptors to maintain their activity towards the analyte; Impact of receptor packing density, orientation of the immobilised receptor, and receptor layer regularity on effectiveness of the analyte recognition process; planning sensor construction for a selected detection method; examples of sensor/biosensor applications; introduction to fundamental norms (ISO) concerning chemical analysis, with a particular focus on elemental and molecular analysis; discussion on the validation stage of analytical methods, validation criteria, and their importance for ensuring result quality; the significance of reference materials in the process of elemental and molecular analysis, preparation, storage, and use procedures for certified reference materials (CRM); practical examples of analysis applications in environmental and clinical contexts, illustrating the importance of adhering to norms and regulations; review of commonly used analytical techniques in elemental analysis (e.g., atomic absorption spectrometry) and molecular analysis (e.g., mass spectrometry) - advantages and limitations.
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
The Student
K_W01 – Has extended knowledge of the place of chemistry in the system of exact sciences
and natural sciences, and about its importance for the development of humanity.
K_W04 – Knows aspects of the construction and operation of modern measuring equipment supporting scientific research in chemistry.
K_W05 – Possesses in-depth knowledge and skills in the selected chemical specialization allowing the use of methods and concepts appropriate to this specialization and allowing for independent research work.
K_W10 – Knows aspects of the construction and operation of modern measuring equipment supporting scientific research in a given chemical specialization.
K_U03 – Is able to apply appropriate methods, techniques and research tools within a given chemical specialty, necessary to explain the given problem.
K_U07 – Is able to critically evaluate the results of independently conducted experiments within his chemical specialty, as well as discuss measurement errors.
K_U09 – Is able to apply the acquired knowledge to related scientific disciplines and work in interdisciplinary teams.
K_U11 – Is able to discuss the place of chemistry in the system of exact and natural sciences and its importance for the development of our civilization.
K_K01 – Understands the need and knows the possibilities of continuous education. Is able to independently search for information in the literature (including foreign languages) and use the advice of experts.
K_K04 – Is convinced of the importance of behaving in a professional manner and follows the principles of professional ethics.
K_K05 – Is able to formulate opinions on professional issues and argue for them both in the environment of specialists and non-specialists
Assessment criteria
Attendance is mandatory; Active participation rewarded;
Final written exam (each part of the lecture must be passed with 50% and the grade is average), oral retake exam;
Permissible number of excused absences is 2 classes.
Bibliography
Specjacja Chemiczna, Problemy i możliwości, pod redakcją Danuty Barałkiewicz i Ewy Bulskiej, MALAMUT, Warszawa 2009;
Współczesna Chemia Analityczna, Wybrane zagadnienia, Adam Hulanicki, PWN 2001;
Sensory chemiczne i biosensory, pod redakcją Zbigniewa Brzózki, Elżbiety Malinowskiej, Wojciecha Wróblewskiego, Wydawnictwo Naukowe PWN, Warszawa 2022.
Metrology in Chemistry, Ewa Bulska, Springer 2018 (English translation of the 2nd original Polish edition published by Wydawnictwo Malamut, Warszawa, 2012)Spektrometria Atomowa. Możliwości analityczne, pod red. Ewy Bulskiej i Krystyny Pyrzyńskiej, Malamut, Warszawa 2007
Handbook of Elemental Speciation, Techniques and Methodology, Rita Cornelis, WILEY 2003;
Fundamentals of Analytical Chemistry by Douglas Skoog, Cangage Learning Inc 2021
AND Materials provided by the instructors.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: