Methods of Organic Compounds Identification B - laboratory 1200-1MIZOBL6

The goal of the course is to learn the ability to:
- use chemical and physical methods of identification of simple organic compounds,
- perform chemical reactions identifying functional groups - determining physical properties of a compound,
- apply chromatography to identify simple organic compounds; choose thin-layer chromatography conditions,
- apply chemical methods to separate components of simple organic compounds and isolate biological material components,
- apply column chromatography to separate components of mixtures of natural compounds (choosing conditions, adsorbents and eluents),
- apply UV-Vis spectroscopy to identify biological material components,
- compare experimental data with literature,
- analyze spectral data, identify compounds basing on infrared (IR) spectra and 1H NMR, 13C NMR nuclear magnetic resonance spectra.

Type of course

obligatory courses

Mode

Classroom

Prerequisites (description)

Knowledge of organic chemistry basics. Furthermore, student should be able to conduct synthesis of simple organic compounds and be proficient with laboratory techniques used in organic chemistry: crystallization, extraction, simple distillation, vacuum distillation, steam distillation. Student should know the laboratory safety rules.

Course coordinators

Joanna Szawkało

Learning outcomes

Upon completing the laboratory student:
- knows theoretical basis for experimental methods utilized in identification of mixture components and determining structures of simple organic compounds,
- knows experimental methods utilized in separation of mixtures of organic compounds,
- knows experimental methods utilized in identification of organic compounds,
- is able to pick separation method and separate a mixture of organic compound by chemical and chromatographic methods,
- chooses conditions for thin-layer and column chromatography,
- is able to use ultraviolet-visible (UV-Vis) spectroscopy method and record and analyze UV-Vis spectra,
- recognizes functional groups basing on infrared spectrum, analyzes IR spectra,
- is able to identify simple organic compounds based on their NMR data, analyzes 1H NMR and 13C NMR spectra, and data of spectrometry mass
- is able to reliably analyze experiment results,
- knows the basic principles of health and safety sufficient to work in a chemical laboratory,
- has the ability to organize own and team work as part of joint tasks and projects, and critically assesses its level of advancement,
- independently undertakes and initiates simple research activities.

Assessment criteria

Mandatory attendance at all classes.
Only 1 absence is allowed which must be made up at another time.

To pass the entrance test, student must earn a minimum of 50% of points. Students who failed, have the opportunity to improve their grade by writing a test of all material (open questions, problems to be solved).
The condition for obtaining a positive grade from the laboratory is to pass the theoretical and practical parts.
The final grade consists of partial grades from:
Tests verifying preparation for class
Exercise execution, written description of exercise, etc.

Practical placement

not applicable

Bibliography

1. O. Demchuk, M. Topyła, K. Kapłon, Ćwiczenia laboratoryjne z identyfikacji związków organicznych, Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej, 2014
2. K. Bańkowski, A. Krawczyk, R. Siciński, J. Stępiński, A. Temeriusz, Ćwiczenia z organicznej analizy jakościowej i chemii bioorganicznej, Wydawnictwo UW, 1990
3. Z. Jerzmanowska, Analiza jakościowa związków organicznych, PZWL, 1975
4. R. Walczyna, J. Sokołowski, G. Kupryszewski, Analiza związków organicznych, Wydawnictwo Uniwersytetu Gdańskiego, 1996
5. A. Vogel, Preparatyka organiczna, WNT, 1984
6. Z. Witkiewicz, Podstawy chromatografii i technik elektromigracyjnych, PWN, 2017
7. Z. Witkiewicz, Podstawy chromatografii, WNT, 2000
8. A. Berthillier, Chromatografia i jej zastosowanie, PWN, 1975
9. M. Szafran, Z. Dega-Szafran, Określanie struktury związków organicznych metodami spektroskopowymi, PWN, 1988
10. R.M. Silverstein, F.X. Webster, D.J. Kiemle, Spektroskopowe metody identyfikacji związków organicznych, PWN, 2007
11. F. Wehrli, T. Wirthlin, Interpretacja widm w spektroskopii 13C NMR, PWN, 1985
12. A. Ejchart, L. Kozerski, Spektroskopia magnetycznego rezonansu jądrowego 13C, PWN, 1988
13. W. Zieliński, A. Rajca (red), Metody spektroskopowe i ich zastosowanie do identyfikacji związków organicznych, WNT, 1995
14. a) R.T. Morrison, R.N. Boyd, Chemia Organiczna, PWN, 1998. b) J. McMurry, Chemia Organiczna, PWN, 2005
15. B.D. Hames, N.M. Hooper, J.D. Houghton, Krótkie wykłady: Biochemia, PWN, 2001
16. R.K. Murray, D.K. Granner, P.A. Mayes, V.W. Rodwell, Biochemia Harpera, PZWL, 2004

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 1200-1MIZOBL6 in USOSweb