Methods of Organic Compounds Identification Laboratory A 1200-1MIZOAL6

The goal of the course is to learn the ability to:
- use chemical and chromatographic methods of identification of simple organic compounds,
- apply chromatography to separate, check purity and identify mixture components,
- record spectra UV-Vis and use UV-Vis spectral data to identify and determine purity of organic compounds and mixture components.
List of exercises:
Exercise 1. Reactions identifying functional groups.
Exercise 2. Identification of unknown organic compound. Independent performance and recording of H NMR spectra of tested organic compounds.
Exercise 3. Application of thin-layer chromatography to identify components of organic compound mixture. Isolation of components from biological material.
Exercise 4. Separation of biological material components by means of column chromatography.
Exercise 5. Identification of biological material components with UV-Vis spectroscopy; comparison of obtained experimental data with literature.
Exercise 6. The choice of conditions for chromatographic analysis.
Determining the composition of the mixture of organic compounds by thin layer chromatography. Identification of the components on the basis of IR, NMR and MS spectra.

Main fields of studies for MISMaP

chemistry

Type of course

obligatory courses

Mode

Classroom

Prerequisites (description)

Knowledge of organic chemistry basics. Furthermore, student should be able to conduct synthesis of simple organic compounds and be proficient with laboratory techniques used in organic chemistry: crystallization, extraction, simple distillation, vacuum distillation, steam distillation. Student should know the laboratory safety rules.

Course coordinators

Katarzyna Pałka

Learning outcomes

Upon completing the course student:
- knows experimental methods utilized in identification of organic compounds,
- knows applications of organic compounds identification methods in biology,
- is able to choose conditions for thin layer and column chromatography,
- is able to use UV-Vis spectroscopy method and record UV-Vis spectra,
- is able to identify simple organic compounds on the basis of IR spectra, NMR and MS,
- knows how to write and interpret the H NMR spectrum of a simple organic compound independently.

Assessment criteria

Mandatory attendance
One excused absence is possible, which should take place in the additional laboratory
Tests verifying preparation for class – 70%
Execution, written description of exercise – 20%
Cooperation in collective exercises,
activity, self-reliance, other – 10%

Practical placement

not applicable

Bibliography

1. K. Bańkowski, A. Krawczyk, R. Siciński, J. Stępiński, A. Temeriusz - Ćwiczenia z organicznej analizy jakościowej i chemii bioorganicznej - Wyd. UW, 1990.
2. A. Vogel - Preparatyka organiczna - WNT, 1984 lub 2006
3. Z. Jerzmanowska - Analiza jakościowa związków organicznych - PZWL, 1975.
4. J. Woliński, J. Terpiński - Organiczna analiza jakościowa - PWN, 1973.
5. J. Bojarski (red) - Ćwiczenia z preparatyki i analizy organicznej - Collegium Medicum - UJ, 1996.
6. R. Walczyna, J. Sokołowski, G. Kupryszewski - Analiza związków organicznych -
Wyd. Uniw. Gdańskiego, 1996.
7. J. Wróbel (red.) - Preparatyka i elementy syntezy organicznej - PWN, 1983.
8. J.A. Moore, D.L. Dalrymple - Ćwiczenia z chemii organicznej - PWN, 1976.
9. A. Berthillier - Chromatografia i jej zastosowanie - PWN, 1975.
10. Opieńska-Blauth, H. Kraczkowski, H. Brzuszkiewicz - Zarys chromatografii cienkowarstwowej - PWRiL, 1971, 1976.
11. L.A. Kazicyna, N.B. Kupletska - Metody spektroskopowe wyznaczania struktury związków organicznych - PWN, 1974, 1976.
12. M. Szafran, Z. Dega-Szafran - Określanie struktury związków organicznych metodami spektroskopowymi - PWN, 1988.
13. a) R. M. Silverstein, C. C. Bassler - Spektroskopowe metody identyfikacji związków organicznych - PWN 1970. b) R. M. Silverstein, F.X. Webster, D.J. Kiemle Spektroskopowe metody identyfikacji związków organicznych - PWN 2007.
14.F. Wehrli, T. Wirthlin - Interpretacja widm w spektroskopii 13C NMR - PWN, 1985
15. A. Ejchart, L. Kozerski - Spektroskopia magnetycznego rezonansu jądrowego 13C - PWN, 1981, 1988.
16. W. Zieliński, A. Rajca (red) - Metody spektroskopowe i ich zastosowanie do identyfikacji związków organicznych - WNT, 1995.
17. C.N.R. Rao, – Spektroskopia elektronowa związków organicznych - PWNT, 1982.
18. a) R.T. Morrison, R.N. Boyd - Chemia Organiczna - PWN, 1998.
b) J. McMurry - Chemia Organiczna - PWN, 2005.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 1200-1MIZOAL6 in USOSweb