Spectroscopy and Biospectroscopy 1200-1CHMSPEW5
The course will begin with an overview of fundamental properties of electromagnetic radiation, empirical observations of absorption, emission and scattering of light and manifestations of these processes in the form of spectra.
Subsequently, the course will touch upon following topics:
• basic methodological concepts of spectra acquisition, parameters characterizing spectral data;
• dipole transition moment;
• infrared absorption spectroscopy: selection rules, harmonic/anharmonic oscillator models, interpretation of spectra;
• rotational spectroscopy;
• Raman scattering;
• electronic optical spectroscopy: selection rules, fluorescence and its quenching;
• single-molecule spectroscopy;
• time-resolved spectroscopy;
• circular dichroism and its applications in studies of chiral biomacromolecules;
• basic principles of NMR and EPR spectroscopy;
• design of NMR spectrometers;
• shielding, chemical shift, spin-spin couplings, relaxation;
• basic features of Fourier transform;
• correlation spectroscopy;
• basic application in chemistry and biochemistry;
• basic principles of MRI.
Main fields of studies for MISMaP
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
1. Knowledge of fundamentals and range of applications of chief methods of molecular spectroscopy.
2. Independent interpretation of vibrational / NMR spectra of various simple chemical compounds.
3. The ability to select adequate spectroscopic methods to routine tasks involving detection and identification of selected types of chemical compounds.
Assessment criteria
Written exam (60 min)
Practical placement
None
Bibliography
1. Kensal E. van Holde, W. Curtis Johnson, P. Shing Ho "Principles of physical biochemistry", Upper Saddle River, NJ, Pearson Education International, 2006.
2. Charles R Cantor, Paul R Schimmel Biophysical Chemistry Part I: The Conformation of Biological Macromolecules; Part II: Techniques for the Study of Biological Structure and Function; Part III: The Behavior of Biological Macromolecules, New York : W. H. Freeman and Company, 1980, 2001, 2002
3. James Keeler. Understanding NMR Spectroscopy, chapter 2-5, 12. John Wiley & Sons, Ltd, Chichester, UK, 1 edition, 2005. Bezpłatna wersja online www-keeler.ch.cam.ac.uk/lectures/
4. Malcolm H. Levitt. Spin dynamics: Basics of Nuclear Magnetic Resonance, chapter 1-5, 8-11, 14-15. John Wiley & Sons, Ltd, Chichester, UK, 2 edition, 2008
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: