Molecular Biology and Biotechnology Laboratory A 1200-1CHMLBMAL5
During the laboratories, exercises will be conducted to show students the possibilities of using molecular biology and biotechnology in order to obtain substances of biological importance, diagnostic systems as well as biomaterials and bionanomaterials. The proposed set of exercises:
Exercise 1, exercise 2: Basic laboratory techniques in the microbiology and biotechnology laboratory. The exercise covers issues related to the principles of sterile work, sterilization of media, tools, and reagents, types of media for the cultivation of microorganisms, methods of inoculation and assessment of the number of cells, monitoring the growth dynamics of microbial culture, methods of storing biological preparations (nucleic acids, proteins, cell preparations) ( cycle of two classes)
Exercise 3: Immunochromatography tests in medical applications. Techniques used: synthesis of reporters (Au nanoparticles), conjugation of ligands/antibodies to reporters, side flow test for selected analytes.
Exercise 4: Using antibodies to identify proteins. Techniques used: chromatographic purification of antibodies, immunoprecipitation.
Exercise 5: Genetic analysis of pathogens (microorganisms). Techniques: bacterial culture, DNA isolation, PCR, DNA electrophoresis, sequencing (theory), phylogenetic analysis of DNA sequences (bioinformatic methods)
Exercise 6, exercise 7: Biotechnological methods of obtaining yeast products used in the pharmaceutical and food industries. Techniques: cultivation of Torula and Saccharomyces cerevisiae yeast cultures, separation of biomacromolecules in the biotechnological industry with the use of classical techniques and dialysis/ultrafiltration, enzymatic conversion of biomacromolecules to obtain raw materials for pharmaceutical and food precursors.
Exercise 8: The use of microorganisms in the synthesis of pharmaceutical intermediates. Techniques: the culture of yeast and bacterial cultures, reactions in bioreactors and reactions using metabolites and enzymes of microbial origin for the synthesis of pharmaceutical intermediates, analysis of the obtained products using analytical and preparative techniques of organic chemistry (chiral HPCL, NMR, MS, TLC, flash chromatography)
Exercise 9: 3D biofilm using bacterial cultures in a hydrogel matrix. Techniques: culturing bacterial cultures, creating hydrogels from bacteria, printing living hydrogels with a 3D printer, survival tests of the obtained material.
Exercise 10, exercise 11: Molecular cloning of a DNA fragment. Techniques: amplification of a DNA fragment or its excision, modification, and pasting into the target vector as well as transformation into host cells and selection. The use of enzymes, PCR techniques and agarose gel electrophoresis
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Has basic knowledge in selected areas of prokaryotic and eukaryotic biotechnology and understands the relationships and dependencies between various biotechnology specialties.
Demonstrates knowledge of the basics of natural sciences leading to the use of living organisms in the preparation of substances and functional materials.
Demonstrates knowledge in the field of using living organisms, in particular genetically modified organisms, to obtain biologically active substances.
Understands the application of immunological mechanisms in the field of disease prevention and detection.
Demonstrates knowledge in the field of obtaining biotechnological products that require additional chemical processes.
Shows knowledge in the field of applying the achievements of biotechnology in tissue engineering, with particular emphasis on 3D bio-printing.
Demonstrates knowledge of basic medical diagnostic methods based on biotechnology, with particular emphasis on detection and imaging methods.
Shows the knowledge of modern methods of nanomaterials application in biotechnology and medicine.
Understands the correlation between molecular biology, biotechnology, in particular medical biotechnology, and organic chemistry and biochemistry.
Understands the correlation between individual biotechnology specialties, in particular between red (medical) biotechnology and green, yellow, and blue biotechnology.
Demonstrates knowledge of the basic vocabulary in the field of biotechnology, in particular medical biotechnology.
Demonstrates the ability to read and understand specialist literature in modern English (English) and to communicate at a basic level.
Demonstrates the ability to use available information sources, including electronic sources.
Shows understanding of biological phenomena and processes essential for the functioning of living organisms, their application in biotechnology, in particular medical biotechnology
Has knowledge of the basic methods used in the identification and characterization of biomolecules and the organization of a research laboratory.
He knows the principles of formulating hypotheses, building models, and formulating theories in the context of the concept of the development of molecular biology and biotechnology.
Is aware of the ethical controversies related to various aspects of molecular biology and biotechnology.
Has advanced knowledge of mathematics allowing for understanding, quantitative description, and/or modeling of chemical and/or biotechnological processes.
He knows the rules of safe work as well as chemical and biological hazards in the research/measurement laboratory.
Assessment criteria
The final grade is the arithmetic mean of partial grades from individual laboratory exercises. Partial grades are obtained by the student on the basis of the entry test and/or the final test for a given exercise; in the latter case, a discussion of the results based on the report may be the basis. During the semester, the student may not participate in one laboratory exercise, which is included in the average of partial grades as grade 0.
Bibliography
"Biologia molekularna w medycynie. Elementy genetyki klinicznej". Redakcja: Jerzy Bal. Wydawca: PWN. Język wydania: polski. ISBN: 978-83-01-16665-6. Rok wydania 2013
"Biotechnologia molekularna. Geneza, przedmiot, perspektywy badań i zastosowań. Wydanie: Warszawa, 1, 2007. Autor: Jerzy Buchowicz. Wydawca: Wydawnictwo Naukowe PWN
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: