Laboratory of general microbiology and bacterial genetics 1100-2BB15
The aim of the Practicum is to present the basic information on bacteria, methods of studies on bacteria and techniques of genetic engineering based on use of bacteria.
Program:
1. Techniques of working in a microbiological laboratory - sterile work, microbiological media.. Isolation of pure bacterial cultures and determination of their number.
2. Morphology and structure of a bacterial cell (description based on the staining).
3. Influence of physical and chemical factors on bacterial growth.
4. Elements of bacterial genetics - plasmids, transformation, coniugation.
5. Regulation of gene expression - lactose operon of E. coli.
Student's amount of work:
The laboratory = 15 hours
Preparation of the reports = 7.5 hours (1.5 hour per report)
Unassisted preparation for the exercise = 7.5 hours
Preparation for the test = 15 hours
Total about 45 hours
Type of course
Mode
Learning outcomes
Having learned the material covered by the classes, the student:
KNOWLEDGE
1. knows the structure of a bacterial cell, structural differences between Gram-positive and Gram-negative bacteria, (K_W06)
2. knows elements of bacterial genetics: plasmids, horizontal gene transfer, (K_W06)
3. knows basic microbiologic techniques. (K_W04)
ABILITIES
1. can work sterile, justify the choice of sterilisation method of solutions, media, glass and small equipment, (K_U01)
2. can isolate pure bacterial cultures and prepare bacterial cultures,(K_U01)
3. can transform bacterial cells with a plasmid vector, (K_U01)
4. can interpret simple results of experiments performed using bacteria. (K_U01, K_U06)
ATTITUDES:
1. is aware of the meaning of research ethics and responsibility for reliability of the obtained results, (K_K04, K_K05)
2. understands the possibilities of using bacteria in biotechnology, medicine, environment protection and in the industry. (K_K06, K_K07)
Assessment criteria
1. Submitting of the completed result cards
2. Written final test in a form of multi-choice test and tasks of short answer.
The final grade is a weighted average of grades for reports (weigh 1/3) and a grade from the final test (weigh 2/3).
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: