Molecular biology with genetics, part I 1100-2BB10
Program:
1. The genome. Size and structure of the genome: comparison of human genome with genomes of other organisms (bacteria, yeast, plants). Chromatin and its different states; nucleosome and its structure. Chromosome structure. Human karyogram. Telomeric DNA. Genes in the genome; gene families. Genetic organisation of the prokaryotic and eukaryotic genome. Operons.
2. Genomics and its variations (functional, comparison, theoretic, environmental, of individual differences, pharmacogenomics), proteomics, transcriptomics, interactomics, metabolomics, bioinformatics, systems’ biology, knowledge of the above mention names.
3. DNA structure and DNA-binding proteins. B-, A- and Z-DNA. Identification of protein-binding sites in the DNA. Participation of different kinds of protein domains in DNA interaction.
4. Mechanisms of prokaryotic and eukaryotic genome replication. Mutagenesis. Repair mechanisms of DNA. Genome evolution pathways.
5. Initiation, elongation and termination of transcription and their regulation in Prokaryotes and Eukaryotes. Maturation of different RNAs; mechanisms. Pre-mRNA splicing with participation of small nuclear RNAs; spliceosome complexes. “Capping” and polyadenylation. RNA editing.
6. Gene expression regulation during intracellular transport of RNA and proteins via nuclear pore complexes.
7. Mechanisms of protein biosynthesis in prokaryotic and eukaryotic organisms. Post-translational processing of the proteome.
8. Mechanisms of enzymatic RNA degradation.
9.Regulation of gene expression with participation of small non-coding RNA molecules (siRNA, miRNA).
Type of course
Mode
Course coordinators
Learning outcomes
KNOWLEDGE:
The student knows the basic problems of a modern molecular biology and genetics.
ABILITIES:
The student can, without assistance, investigate the latest achievements in the field of the subject.
ATTITUDES:
The student understands the meaning of problems being a part of the course for medicine and ethics.
He / she can develop, without assistance, certain attitudes towards modern research challenges in the field of molecular genetics.
Assessment criteria
Exam in a written form, in doubtful cases also in oral form. Possibility of passing the exam on the basis of two tests: one in the middle of the semester, the second at the end of the semester.
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: