Techniques of Molecular Biology in Forensics 4027-KNS-BG-23-TBM
Organization and chemical composition of the cell and the human body. Structure and properties of basic organic compounds found in living organisms: proteins, nucleic acids, sugars and lipids. Structure and properties of enzymes and antibodies. Nuclear and mitochondrial genome. DNA replication. Gene structure. Mutations and polymorphisms. DNA isolation from human material. Nucleic acids electrophoresis. DNA cloning. DNA sequencing. Hybridization of nucleic acids. PCR. Protein electrophoresis. Immunoblotting, ELISA, immunoprecipitation and agglutination. VIS and UV spectrophotometry. Spectrofluorimetry. Chromatography. Mass spectrometry.
Type of course
Mode
Course coordinators
Learning outcomes
The student has a basic knowledge of molecular biology. Knows and understands the molecular basis of cell functioning. Can apply techniques of molecular biology and conduct basic experiments in this field. Can make logical inferences based on the obtained results. Is able to cooperate with other experts, basing on the results of the own research.
Assessment criteria
A written exam; in order to take the exam, it is necessary to pass the laboratory.
Bibliography
Anna Lewandowska-Ronnegren "Techniki laboratoryjne w biologii molekularnej", MedPharm Polska 2018.
Additional information
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