Cytometry - applications in biology research 1400-228CM
list of topics:
1. Introduction to cytometry - how it works.
2. Characteristic of antibodies, fluorochromes and fluorescent proteins.
3. Flow and image cytometry instrumentation.
4. Preparation of samples for cytometric analysis.
5. Analysis of expression of surface and intracellular markers.
6. Analysis of cell cycle cell, apoptosis and necrosis.
7. Other application of flow cytometry in biological study.
8. Clinical application of flow cytometry.
9. Application of image cytometry
10. Cell sorting.
11. Overview of data processing and advanced multiplex analysis.
12. Data presentation.
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Knowledge
1. Is familiar with theoretical basis of flow cytometer functioning and samples designing. (K_W12)
2. Knows basic differences in various types cytometers and sorters operation and softwares. (K_W10)
3. Has broad knowledge in application of flow cytometry for phenotypical and functional analysis of immune cells. (K_W13)
4. Has knowledge in different application of flow cytometry for analysis of prokaryotic and eukaryotic cells. (K_W10)
Skills
1 .Ability to use cytometers FACSCalibur and Verse, sorter FACSAria , High Content Imaging device Pathway. (K_U01)
2 .Ability to prepare cells for flow cytometric acquisition and analysis.(K_U01)
3. Ability to use CellQuest, Diva and FACSuite software for cell analysis. (K_U01)
4. Is able to plan experiments, draw proper conclusions and interpretation of research results based on the obtained data under control of supervisor. (K_U07)
Social skills
1. Is responsible for the organization of safety conditions for conducted experiments and is able to evaluate the level of hazard resulting from experimental techniques. (K_K08)
2. Is responsible for the research which has been assigned to him/her, for his/her own laboratory work and other people’s work. (K_K04)
Assessment criteria
Verification of practical skills during classes. Assessment in the form of a multimedia presentation on selected cytometric methods with oral verification of knowledge during the presentation.
Practical placement
Not required.
Bibliography
1. Practical Flow cytometry H. M. Shapiro, 2003 Wiley-liss
2. Current protocols in cytometry, Wiley
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: