Animal metabolism regulation 1400-227RMZ

THIS COURSE IS THE EQUIVALENT OF THE FORMER COURSE "METABOLISM REGULATION".

The aims of the lecture are as following:

- presentation of metabolic regulation as a problem of vital importance,
- discussion of methods for determination of changes in enzyme content and enzyme activity
- identification of the inborn metabolic errors and the regulatory steps in a metabolic pathway,
- elucidation of the mechanisms of hormone-induced signal transductions, particularly those evoked by steroid and peptide hormones,
- explanation of importance of "cascade system" in the hormone action on cellular metabolism,
- presentation of the subcellular localization of metabolic processes and the regulatory mechanisms of metabolite transport through the cellular and subcellular membranes.

During the classes, performed as individual laboratory work, the students will deal with the following tasks:

1. Glycolytic activity in brain. The effect of ATP and NAD+ deficit on glycolytic activity. Product (lacatate) to substrate (glucose) ratio. Spectrophotometric determinations.
2. Metabolite transport through mitochondrial membrane. The measurement of the activity of the transport based on the phenomenon of mitochondria swelling in isotonic solutions.
3. Arginine metabolism in mitochondria. The effect of various factors (e.g. 2-oxoglutarate, transamination inhibitors) on the metabolism of one of proteinogenic amino acids. RP-HPLC application for amino acids concentration measurements in biological material.
4. Glycogen metabolism in liver. The effect of various factors on the activity of glycogen phosphorylase. Designing experiments using inhibitors of non-specific reactions. Spectrophotometric measurements.
5. (Theoretical task) Pyruvate metabolism in liver. The pathways of pyruvate metabolism. Interpretation of the results performed by other researchers. Designing complementary experiments verifying the formulated hypothesis.