Cell Ultrastructure - Techniques and Methods 1400-225USTR
Ultrastructure of eukaryotic and prokaryotic cells. Ultrastructure of eukaryotic cell: cell membranes, organelles, cytoskeleton; ultrastructure of animal tissues (epithelial tissue, muscle tissue, connective tissue - e.g. blood cells, nerve tissue); ultrastructure of plant tissues (e.g. epidermis, parenchyma, sclerenchyma, colenchyma, phloem, xylem); ultrastructure of fungi cells: e.g. ultrastructure of cell wall; ultrastructure of protist cells. Ultrastructure of prokaryotic cell (e.g. organelles, cell wall).
Methods and technics of electron microscopy: fixatives and fixation methods, dehydrating agents and dehydratation, embedding media and embedding methods (eg. epoxy and polyester resins; blocks and glass knifes preparation; microtomes, cutting of thin and semithin sections; staining methods for sectioned material; examination and analysis of biological specimens in SEM and TEM; electronograms praeparation and analysis.
Type of course
Mode
Course coordinators
Learning outcomes
Knowledge:
Graduates have an in-depth knowledge and understanding of the rules and molecular, cellular and physiological mechanisms of the development and functioning of organisms (S2_K_W05).
Graduates know and understand specialist concepts in biochemical and microbiological terminology, the biology of fungi, plants and animals, genetics, molecular biology and environmental biology, as well as specialist literature in these areas (S2_K_W06);
Graduates know and understand in depth the various research techniques and tools used in the biological sciences and plan their use appropriately to solve the tasks set (S2_K_W10);
Graduates know and understand advanced laboratory, measurement and imaging techniques used in biological research (S2_K_W12);
Graduates know and understand the principles of planning research and conducting experiments using specialist methods applied in the studied specialisation of biological sciences (S2_K_W13);
Graduates know and understand the principles of occupational health and safety and ergonomics, ensuring safe work in the laboratory, including work with radioactive substances (S2_K_W16);
Skills:
The graduate is able to apply research techniques and tools appropriate to the problems of the studied specialisation in biological sciences (S2_K_U01); Graduates are able to plan and carry out experiments using the methods they have learned under the supervision of a scientific supervisor; they are able to propose methods for carrying out specific tests and assess the usefulness of the methods and their limitations for the material under study (S2_K_U07);
Graduates are able to critically analyse a selected biological problem on the basis of literature data and the results of their own research, formulating their own opinions and conclusions (S2_K_U08).
Competences:
Graduates are prepared to share knowledge about the latest achievements in natural sciences with the public and to explain the rationale behind conducting basic scientific research (S2_K_K03).
Graduates are prepared to continuously improve their skills and update their knowledge by using scientific and popular science sources related to specialist biological sciences (S2_K_K07);
The graduate is prepared to demonstrate responsibility for assessing the risks associated with the research techniques used and to create ergonomic and safe working conditions (S2_K_K08).
Assessment criteria
Lecture – written examination in the form of a test consisting of single-choice questions and open-ended questions with short answers.
Laboratories – written assessment in the form of a final paper describing the results obtained during the classes.
Practical placement
not applicable
Bibliography
1.Barbacki A., Czajka R., Jóźwiak K., Kachlicki T., Mikroskopia elektronowa, Wydawnictwo Politechniki Warszawskiej, Poznań, 2005;*
2.Bielańska - Osuchowska Z., Kawiak J. (red), Struktura funkcjonalna komórek i tkanek, PWN, 1991 (także wydania późniejsze);
3.Bloom W., Fawcet D.W., Histologia, PZWL, 1967 (lepsza wersja angielska: Textbook of histology, Sauders W.B. (ed.) 2006;
4.Cieciura J. (red) Techniki stosowane w mikroskopii elektronowej, PWN, 1989;
5.Czubaj A., Sobolewska M., Ultrastruktura komórki - metody i techniki mikroskoppii elektronowej, skrypt Prac. Mikr. Elektronowej, 2005;
6.Czub P., Bończa - Tomaszewski Z., Penczek P., Pielichowski J., Chemia i technologia żywic epoksydowych, Wydawnictwo Naukowo-Techniczne, Warszawa, 1960, 2002;
7.Jasiński A., Kilarski W., Atlas ultrastruktury komórek kręgowców, PWN, 1981;
8.Jasiński A., Kilarski W., Ultrastruktura komórki, WSiP, 1984;
9.Krzywy T., Ślopek S., Morfologia i ultrastruktura bakteriofagów Shigella i Klebsiella, PZWL:, 1974;
10.Ostrowski K., (red.). Histologia, PZWL, 1988;
11.Pawlikowski M., Kryształy w organizmie człowieka, Wydawnictwo i Drukarnia "Secesja", Kraków, 1993;
12.Sawicki W., Histologia, PZWL, 1993,1997;
13.Sobotta., Histologia. Kolorowy atlas cytologii i histologii człowieka. Urban i Partner., 1998;
14.Sobotta, Atlas histologii, (Welsch U. ed.)Urban & Partner., 2002;
15.Stevens A., Lowe J. Histologia, WDSV (ed), 1994;
16.Stevens A., Lowe J. Histologia człowieka, PZWL, SV (ed), 2002;
17.Wróbel B., Zienkiewicz K., Smoliński J. D., Niedojadło J., Świdziński M., Podstawy Mikroskopii Elektronowej; Wyd. UMK, Toruń, 2005
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: