Cell Ultrastructure - Techniques and Methods 1400-225USTR
Ultrastructure of eukaryotic and prokaryotic cells. Ultrastructure of eukaryotic cell: cell membranes, organelles, cytoskeleton; ultrastructure of animal tissues (epithelial tissue, muscle tissue, connective tissue - e.g. blood cells, nerve tissue); ultrastructure of plant tissues (e.g. epidermis, parenchyma, sclerenchyma, colenchyma, phloem, xylem); ultrastructure of fungi cells: e.g. ultrastructure of cell wall; ultrastructure of protist cells. Ultrastructure of prokaryotic cell (e.g. organelles, cell wall).
Methods and technics of electron microscopy: fixatives and fixation methods, dehydrating agents and dehydratation, embedding media and embedding methods (eg. epoxy and polyester resins; blocks and glass knifes preparation; microtomes, cutting of thin and semithin sections; staining methods for sectioned material; examination and analysis of biological specimens in SEM and TEM; electronograms praeparation and analysis.
Type of course
Mode
Course coordinators
Learning outcomes
Knowledge:
He knows in an in-depth extent the ultrastructural, structural and functional diversity of living organisms (S3_W01); The rules of proper planning of ultra-structural studies and the use of individual techniques for solving set tasks will be given (S3_W03); A variety of techniques and advanced research tools used in studies of ultrastructure of pro- and eukaryotic organisms (S3_W04)
Skills:
Is able to properly select and apply research techniques and methods depending on the needs of the experiment (S3_U01); Is able to make a critical evaluation of the results obtained on the basis of possessed and acquired knowledge (S3_U01); Creates under the supervision of the supervisor and independently documented studies (S3_U02)
competence:
Exhibits responsibility for threats resulting from applied research techniques (S3_K01); He can work in a team, carrying out his own research, co-organizing the work of the whole team (S3_K03)
Assessment criteria
Lecture - exam, open questions
Classes - final work
Practical placement
not applicable
Bibliography
1.Barbacki A., Czajka R., Jóźwiak K., Kachlicki T., Mikroskopia elektronowa, Wydawnictwo Politechniki Warszawskiej, Poznań, 2005;*
2.Bielańska - Osuchowska Z., Kawiak J. (red), Struktura funkcjonalna komórek i tkanek, PWN, 1991 (także wydania późniejsze);
3.Bloom W., Fawcet D.W., Histologia, PZWL, 1967 (lepsza wersja angielska: Textbook of histology, Sauders W.B. (ed.) 2006;
4.Cieciura J. (red) Techniki stosowane w mikroskopii elektronowej, PWN, 1989;
5.Czubaj A., Sobolewska M., Ultrastruktura komórki - metody i techniki mikroskoppii elektronowej, skrypt Prac. Mikr. Elektronowej, 2005;
6.Czub P., Bończa - Tomaszewski Z., Penczek P., Pielichowski J., Chemia i technologia żywic epoksydowych, Wydawnictwo Naukowo-Techniczne, Warszawa, 1960, 2002;
7.Jasiński A., Kilarski W., Atlas ultrastruktury komórek kręgowców, PWN, 1981;
8.Jasiński A., Kilarski W., Ultrastruktura komórki, WSiP, 1984;
9.Krzywy T., Ślopek S., Morfologia i ultrastruktura bakteriofagów Shigella i Klebsiella, PZWL:, 1974;
10.Ostrowski K., (red.). Histologia, PZWL, 1988;
11.Pawlikowski M., Kryształy w organizmie człowieka, Wydawnictwo i Drukarnia "Secesja", Kraków, 1993;
12.Sawicki W., Histologia, PZWL, 1993,1997;
13.Sobotta., Histologia. Kolorowy atlas cytologii i histologii człowieka. Urban i Partner., 1998;
14.Sobotta, Atlas histologii, (Welsch U. ed.)Urban & Partner., 2002;
15.Stevens A., Lowe J. Histologia, WDSV (ed), 1994;
16.Stevens A., Lowe J. Histologia człowieka, PZWL, SV (ed), 2002;
17.Wróbel B., Zienkiewicz K., Smoliński J. D., Niedojadło J., Świdziński M., Podstawy Mikroskopii Elektronowej; Wyd. UMK, Toruń, 2005
Additional information
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