Proteomics 1400-225PROT
In this course students get acquainted with the proteomic methodology. In practical part students will purify protein complexes by Tandem Affinity Purification method and analyze there composition using Mass Spectrometry. In theoretical part students will gain knowledge about various applications of Mass Spectrometry in biology and medicine. This includes the application of mass spectrometry for the identification of proteins and their posttranslational modifications, the information on typical protocols of sample fractionation and preparation, types of mass spectrometric experiments used (including data dependent MS/MS experiment as a basis of modern proteomic analysis) and software tools for the data analysis (database search) and verification steps. Lecture is illustrated with the examples of application of proteomic methodology for solving various problems of bio-medical sciences.
Type of course
Course coordinators
Bibliography
Gary Siuzdak (1996) Mass Spectrometry for Biotechnology, Academic Press (ISBN 0-12-647471-0) - brak polskiej pozycji dot. proteomiki.
E. de Hoffmann, J. Chatette, V. Stroobant (1994) Spectrométrie de masse; Masson Editeur, Paris; tłum. L. Konopski (1998) Spektrometria Mas; Wydawnictwa Naukowo-Techniczne (ISBN 83-204-2291-4).
Robert A. W. Johnstone, Malcolm E. Rosse (1996) Mass spectrometry for chemists and biochemists; Press Syndicate of the University of Cambridge; tłum. pod red. Krystyny Jurkowskiej (2001) Spektrometria mas; Wydawnictwo Naukowe PWN SA (ISBN 83-01-13605-7).
Notes
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Term 2023Z:
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