Basics of animal cell culture for biotechnologists 1400-216PHKz
Lecture:
1. Why do we culture plant cells and tissues? In vitro plant culture - working conditions
2. Totipotentiality of plant cells and organogenesis. Hormonal regulation of morphogenesis
3. Types of plant cell and tissue culture in vitro
4. Somatic embryogenesis and somaclonal variability.
5. Production of secondary metabolites in bioreactors. Biofarming.
6.The cell and tissue culture laboratory equipment
7. The medium and terms of cell culture, and methods of animal cell cultures
8. The three dimensional cell and tissue cultures
9. Obtaining of primary cell cultures and cell lines; Application of cell and tissue cultures in science, biotechnology and medicine
10. Semi-technique scale cultures
Detailed description of laboratory:
1. Organization of laboratory of cells cultures; preparing materials and solutions for cell cultures; sterilization
2. Cultures of cell lines. Cell culture of not adherent cells and adherent cells
- freezing procedure and recovery of cells from liquid nitrogen
- measurement of survival rate
- measurement of cells growth in culture
- passage of adherent and not adherent cells
- Giemsa-MayGrunwald staning
- immunocytochemical localization of cytoskeletal proteins, confocal microscopy
3. Obtaining of primary cell culture from chicken embryo
- culture of fibroblasts, myocytes and retina cells
- Giemsa-MayGrunwald staining of explants
- differentiation of retina cells - Karnowski-Roots reaction
4. Culture of myoblasts cell line C2C12
- freezing of C2C12 cells and recovery of cells from liquid nitrogen
- observation of myoblasts diffrentiation
- Giemsa-MayGrunwald staining
- measurement of cells fusion index
- analysis of cell differentiation in vitro on the basis of myogenic regulatory factors expression by qPCR.
5. Culture and differentiation of mesenchymal stem cells
- culture of non-differentiated cells
- induction of adipocyte differentiation or osteoblasts
- Giemsa-May-Grunwald staining
- evaluation of cell differentiation into adipocytes or osteoblasts, confocal microscopy
6. Summary of results
7. Test
Main fields of studies for MISMaP
Type of course
Mode
Requirements
Prerequisites
Prerequisites (description)
Learning outcomes
Having completed the course and the lab student:
Knowledge
1. Has elementary knowledge in selected basic areas of cell culture in biotechnology and understands the relationships and dependencies between different disciplines of life science.
2. Has knowledge about the cell culture basics in life sciences, conceptual categories and terminology related to cell culture and knowledge about the research methods, and also is able to point out the most important scientific discoveries in the history of biotechnology, including in vitro cultures.
3. Has knowledge about basic cell culture techniques and tools in the study of life science and understands the importance of experimental work in biotechnology and is able to describe the importance of molecular analyzes in biological and medical research.
4. Has knowledge about the technological aspects of cell culture in biotechnology.
Skills
1. Applies basic techniques, appropriate for biotechnology, including in vitro cultures.
2. Shows the ability to use available sources of information, including electronic sources.
3. Carries out simple research tasks under the supervision of a tutor.
4. Shows the ability to acquire and characterize biological material.
Social competence
1. Student understands the biological phenomena and processes in nature.
2. Student is responsible for his own work and equipment; respects self and others work.
3. Student shows the ability to work effectively in a team.
4. Understands the need to provide information about new developments related to cell culture in biotechnology and can provide this information in an understandable way.
Assessment criteria
Lecture - exam. Only students who have completed laboratories can take the exam. The criterion is to show that the knowledge conveyed during the lecture has been lerned.
Laboratories - oral colloquium. Completion of laboratories takes the form of an oral colloquium. The criterion is to show that the knowledge passed during classes has been learned. The condition to take part in the colloquium is passing the practical part of the exercise. The exercise is passed if: the student was present and worked satisfactorily, i.e. she/he completed all the required tasks. The teacher decides about the passing of the exercises.
Practical placement
No
Bibliography
Hodowla komórek i tkanek; Stanisława Stokłosowa; PWN
Cell culture for biochemists; R.L.P. Adams; Elsevier
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: