Bacterial and Archaeal Genetics 1400-216GENBA
During the lecture, the following topics are covered:
-Genetic organization of prokaryotic genomes (DNA, genes and operons, structure of bacterial and archaeal chromosomes).
-Replication and segregation of prokaryotic chromosomes.
-Transcription in bacteria and archaea (types and functions of RNA, transcription mechanisms).
-Translation in bacteria and archaea (genetic code, ribosomes – structure and function, translation mechanisms).
-Regulation of gene expression in bacteria and archaea (levels of gene expression regulation, global gene expression regulation systems – regulons and sigmulons, two-component systems, riboregulation).
-Mutations and DNA repair (types of mutations and mutagenic factors, DNA damage repair mechanisms, SOS regulon).
-Genomic variability in prokaryotes (mobile genetic elements in bacteria and archaea, horizontal gene transfer).
-Bacteriophages.
-Genomics and metagenomics (DNA sequencing methodologies using various techniques, genome assembly and annotation of prokaryotic genomes, metagenomic data analysis).
-Transcriptomics and metatranscriptomics (research methodologies, applications in medical and environmental studies).
-Proteomics, metabolomics, and metabolic modeling.
-Molecular identification and molecular typing of prokaryotic microorganisms.
-Bacterial genetics in industry, medicine, and environmental protection.
-Intellectual property protection in microbiology.
During the exercises, the following topics are addressed:
-Molecular identification of bacteria and archaea.
-Bioinformatics analysis of prokaryotic genomes.
-Gene cloning and recombinant clone selection, techniques for DNA introduction into bacterial cells.
-Overexpression, purification, and study of protein-DNA interactions through electrophoretic mobility shift assays (EMSA).
-Studying protein-protein interactions by examining the ability to form multimeric complexes via cross-linking reactions using glutaraldehyde.
-Investigation of gene expression regulation induced by environmental factors.
-Mutagenesis.
-Molecular epidemiology.
Main fields of studies for MISMaP
biotechnology
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Learning outcomes:
After mastering the material covered in the lectures and exercises, the student:
KNOWLEDGE
-Understands microbiological diversity and the natural phenomena and processes that shape it. Can identify prokaryotic microorganisms using molecular techniques (K_W09 Bl1).
-Understands phylogenetic relationships among microorganisms. Knows methodologies used to determine evolutionary relationships between genes and organisms (K_W10 Bl1).
-Knows laboratory, measurement, and imaging techniques used in microbiological, genetic, and molecular biology research, with particular focus on the genetics of bacteria and archaea (K_W14 Bl1, K_W10 Bl2, K_W12 Bl2, K_W04 Bt1).
-Understands the rules and molecular, cellular, and physiological mechanisms underlying the development and functioning of microorganisms (K_W05 Bl2).
-Is familiar with specialized bioinformatics tools necessary for solving problems in microbial genetics. Can collect and process data using various research and bioinformatics tools and perform complex analytical operations using commonly available computational tools (K_W09 Bl2, K_W14 Bt2).
-Knows the principles of planning research and conducting experiments using specialized methods applied in prokaryotic microbial genetics. Has knowledge of preparing results in a form suitable for discussion, evaluation, or publication (K_W13 Bl2, K_W05 Bt2, K_W08 Bt2).
-Understands the basics of computer science techniques and uses IT tools for information retrieval, text processing, and presentation (K_W08 Bt1).
SKILLS
-Can analyze obtained results and discuss them based on available literature in the field of microbial genetics (K_U03 Bl1).
-Can operate a computer and electronic resources to perform statistical analyses and to locate and use Free Software (K_U04 Bl1).
-Applies appropriate statistical methods as well as computational algorithms and IT techniques to describe phenomena and analyze biological data (K_U06 Bt2).
-Independently plans and conducts research tasks or assessments in the field of microbial genetics (K_U04 Bt1, K_U04 Bt2).
-Demonstrates the ability to critically analyze and select information, especially from electronic sources (K_U03 Bt2).
-Shows the ability to draw conclusions and formulate judgments based on data from various sources (K_U07 Bt2).
Assessment criteria
Laboratory classes (exercises)
The laboratory classes are passed if the student:
-Participated in at least 85% of the classes;
-Worked in a manner that allows a positive evaluation of the knowledge, skills, and social competences acquired during the course (as described in the syllabus as subject-specific learning outcomes);
-Passed a written exam with a minimum score of 51% of the total points available on the test, which consists of open and multiple-choice questions.
Practical placement
Non applicable.
Bibliography
1. Baj J.(red.) "Mikrobiologia", PWN, 2018.
2. Baj J., Markiewicz Z. (red. ) „Biologia molekularna bakterii”, PWN, 2015.
3. Węgleński P. (red.) „Genetyka molekularna”, PWN, 2006.
4. Brown T.A. (red.) „Genomy” PWN , 2009 .
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: