Plasmids and other mobile genetic elements in bacteria - W 1400-215PLAZB-W
I. Modular structure of mobile genetic elements (MGE). Genomics of bacteria. Role of MGE in shaping the structure of bacterial genomes. II. Integrons and supeintegrons (genetic structure, classification, integrons in determination of antibiotic resistance. III. Transposable elements (TE) - characterization of insertion sequences and transposons (classification, distribution of TEs in bacterial genomes, regulation of frequency of transposition, TEs in horizontal gene transfer (HGT). Identification of functional TEs with application of entrapment vectors. IV. Integrative and conjugative elements (ICE). V. Bacterial plasmids (discovery, definition, naming, classification, structure and spread). (1) Plasmid identification (genetic and physicochemical methods). (2). Typical scheme of plasmid characterization (size, copy number, host range and incompatibility group determination, restriction mapping). (2) Plasmid replication (the models of circular and linear plasmid replication; definition of: replicon, minimal vs basic replicon; origin of replication, oriV, mechanisms of replication initiation and regulation; molecular mechanisms of plasmids incompatibility. (3) Stable maintenance of plasmids in bacterial cells (partitioning systems, multimer resolution systems, toxin-antitoxin systems - structure, function and origin). (5) Plasmid conjugal transfer (selftrasmissive and mobilizable plasmids, structure of origin of conjugational transfer (oriT). VI. Phenotypic traits encoded by MGE. VII. Origin and evolution of MGE. VIII. Role of MGEs in horizontal gene transfer. IX. MGEs in genetic engineering and biotechnology.
Type of course
Mode
Course coordinators
Learning outcomes
Having completed the course the student:
KNOWLEDGE
- Has broad knowledge in the fields of biotechnology, molecular biology and genetic engineering of microorganisms, focusing on the mobile genetic elements. (K_W06 Bl2; K_W01 Bt1; K_W02 Bt1; K_W02 Bt2)
- Has up-to-date knowledge in the main areas of bacterial genetics including the scientific terminology concerning microbiology, genetics and genomics, the most recent researches, discoveries and applications of mobile genetic elements in biotechnology, medicine and molecular biology. (K_W02 Bl2; K_W06 Bt1; K_W03 Bt2)
- Is familiar with the rules of research planning, up-to-date techniques of data collection and the use of various research tools, including a broad range of plasmid vectors. (K_W01 Bl2; K_W10 Bl2)
- Has the knowledge of the cellular and molecular basis for microorganism functions in light of the research on bacterial mobile genetic elements. (K_W03 Bl2)
ABILITIES
- Has knowledge of a modern language (Polish or English) sufficient for using electronic resources and scientific literature devoted to broadly defined bacterial genetics. (K_U03 Bl2; K_U02 Bt1; K_U02 Bt2)
- Is able to study on his/her own and focus on the study area. (K_U03 Bt1; K_U03 Bt2)
SOCIAL AWARENESS
- Is careful and critical when acquiring and interpreting knowledge in microbial genetics and its practical applications. (K_K10 Bl2; K_K04 Bt2)
- Understands the need for informing the public about latest achievements in biology of microorganisms and mobile genetic elements and is able to pass this knowledge in an intelligible way. (K_K01 Bl2; K_K02 Bl2; K_K03 Bl2; K_K06 Bt1)
Assessment criteria
The conditions obligatory for passing the final exam:
- Passing the exam in a form of multiple choice test (20) and descriptive exam (10), i.e. gaining at least 51% of points.
Practical placement
No.
Bibliography
1. Biologia Molekularna Bakterii. Baj J., Markiewicz Z. (red.) PWN, 2015
2. Plasmid Biology. Funnel B.E., Philips G.J. (red.) 2003
3. The Horizontal Gene Pool: Bacterial Plasmids and Gene Spread. Thomas C.M. (red.). 2000
4. Mobile DNA III. Craig N.L., Chandler M., Gellert M., Lambowitz A.M., Rice P.A., Sandmeyer S.B. (red.) 2015
5. Bacterial Integrative Mobile Genetic Elements. Roberts A.P. and Mullany P. (red.) 2013
Notes
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Term 2023Z:
None |
Term 2024Z:
None |
Additional information
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