Plant Tissue Culture in vitro 1400-215KTKR
The lecture allow students to acquire basic knowledge in the area of in vitro plant tissue cultures.
Lecture topics:
1. Past and present of plant cultures in vitro. Equipment of plant culture laboratory, media, sterility.
2. Totipotency and hormonal regulation of organogenesis in plants.
3. Types of plant cell and tissue cultures in vitro.
4. Somatic embryogenesis. Somaclonal variation.
5. Somatic hybrid plants – protoplast fusion.
6. Bioreactors and production of secondary metabolites. Culture biotisation.
7. Plant-made pharmaceuticals (PMP). Biofarming.
During laboratory students are acquainted with the basic skills and different kinds of cultures. They use stem, root, hypocotyl, cotyledon and leaf explants to study direct and indirect organogenesis, vegetative micropropagation and somatic embryogenesis on solid and liquid media. For this purpose plant hormones are used. Orchid plants and Gentiana cell culture are used for induction of direct or indirect somatic embryogenesis. The culture of BY-2 serves as an example to study the growth curve of a cell suspension culture and the viability of the cells in particular stages of the culture. Students learn how to obtain somatic hybrid cells by protoplast fusion. In order to preserve of live plant tissue cultures students practise the encapsulation of somatic embryos and a fern gametophytes and their cryopreservation in liquid nitrogen (-196 °C).
Main fields of studies for MISMaP
biotechnology
environmental protection
Type of course
obligatory courses
optional courses
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
KNOWLEDGE: The graduate knows and understands
• in an advanced manner, selected areas of plant biotechnology and cell engineering (K_W02 Bt)
• in depth, the principles of research planning, modern data collection techniques, and the application of various research tools (K_W05 Bt)
• detailed laboratory and industrial procedures applied in plant biotechnology (K_W10 Bt)
• in an advanced manner, the rules as well as the molecular, cellular, and physiological mechanisms of development and functioning of plant organisms (K_W05 Bi)
• advanced laboratory, measurement, and imaging techniques used in biological research (K_W12 Bi)
• the principles of planning research and conducting experiments using specialized methods applied in the studied specialization of biological sciences (K_W13 Bi)
SKILLS: The graduate is able to
• apply research techniques and tools appropriate to problems in the studied specialization of biological sciences (K_U01 Bi, K_U1 Bt)
• identify probable causes of experimental failure using professional literature and appropriately modify the course of the experiment (K_U05 Bi)
• under the supervision of a scientific advisor, plan and conduct an experiment using acquired methods; is able to propose methods for carrying out specified determinations and assess the usefulness and limitations of these methods for the material under study (K_U07 Bi, K_U10 Bt)
• critically present research work in the field of the selected specialization of biological sciences using verbal communication and multimedia (K_U09 Bi)
• work in a team and lead the work of a small group (K_U10 Bt)
SOCIAL COMPETENCIES: The graduate is prepared to
• define priorities for a given task in the field of biological sciences (K_K05 Bi)
• continuously improve and update knowledge by using scientific and popular-scientific sources related to specialized biological sciences (K_K07 Bi)
• demonstrate responsibility for assessing risks associated with research techniques used and for creating ergonomic and safe working conditions (K_K08 Bi)
• critically analyze information appearing in the mass media and apply it in practice (K_K10 Bi)
• demonstrate responsibility for the entrusted scope of research work, as well as for their own and others' work (K_K03 Bt)
• use objective sources of scientific information and apply the principles of critical reasoning when solving practical problems (K_K04 Bt)
Assessment criteria
Laboratory - multimedial presentation. No more than single absence is allowed during laboratory exercises
Lecture - written exam (open questions, test) in which knowledge presented during the lecture and gained during the laboratory will be evaluate.
Practical placement
none
Bibliography
1.Biotechnologia roślin (red. Stefan Malepszy, Warszawa, PWN 2001),
2.Biotechnologia molekularna (Jerzy Buchowicz, Warszawa PWN 2006),
3.Komórki roślinne w warunkach stresu. Tom II (red. Adam Woźny i Krystyna Przybył, Wydawnictwo Naukowe UAM, Poznań 2004).
4.Plant Tissue Culture Techniques and Experiments ( Roberta H. Smith, Academic Press, ISBN #0-12-650342-7).
5. Laboratory Manual (http://www.biol.uw.edu.pl/ibebr/dydaktyka)
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: