Plant Tissue Culture in vitro 1400-215KTKR
The lecture allow students to acquire basic knowledge in the area of in vitro plant tissue cultures.
Lecture topics:
1. Past and present of plant cultures in vitro. Equipment of plant culture laboratory, media, sterility.
2. Totipotency and hormonal regulation of organogenesis in plants.
3. Types of plant cell and tissue cultures in vitro.
4. Somatic embryogenesis. Somaclonal variation.
5. Somatic hybrid plants – protoplast fusion.
6. Bioreactors and production of secondary metabolites. Culture biotisation.
7. Plant-made pharmaceuticals (PMP). Biofarming.
During laboratory students are acquainted with the basic skills and different kinds of cultures. They use stem, root, hypocotyl, cotyledon and leaf explants to study direct and indirect organogenesis, vegetative micropropagation and somatic embryogenesis on solid and liquid media. For this purpose plant hormones are used. Orchid plants and Gentiana cell culture are used for induction of direct or indirect somatic embryogenesis. The culture of BY-2 serves as an example to study the growth curve of a cell suspension culture and the viability of the cells in particular stages of the culture. Students learn how to obtain somatic hybrid cells by protoplast fusion. In order to preserve of live plant tissue cultures students practise the encapsulation of somatic embryos and a fern gametophytes and their cryopreservation in liquid nitrogen (-196 °C).
Main fields of studies for MISMaP
biotechnology
environmental protection
Type of course
obligatory courses
optional courses
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
KNOWLEDGE
1. Student knows functioning of cell structures and important relations between cell components, cells themselves and the whole plant organism (K_W05 BI, K_W06Bi I)
2. Knows biochemical, microbiological, plant biology, molecular and environmental biology terminologies , basic literature as well as practical apllications of plant tissue culture (K_W13 BI I, K_W06 BIII, K_W19 BI II).
3. Understands molecular, cellular and physiological rules of development and functioning of plant organisms (K_W05 BI I).
4. Knows rules of planning and conducting of experiments with the use of specific methods applied in vitro cultures (K_W13 BI II).
5. Is aware about costs of carrying research using methods in vitro and the most important sources of financing of such research ( K_W21 BI I, K_W16 BI II).
6. Posseses knowledge about safety and ergonomy rules, ensuring safe and sterile work at the laboratory applying in vitro technics (K_W21 BI I, K_W16 BI II, K_W19 BI II).
SKILLS
1. Uses basic methods and research tools applied in tissue cultures in vitro and is able to disclose the rules of their functioning (K_U01 BI I.
2. Shows ability of critical analysis and selection of biological informations, especially from electronic sources and media (KU_04 BI I).
3. Is able to analyse received results and using pertinent literature disclose possible causes of unsuccesfull experiments and next properly modify it (K_U04 BI I, K_U05 BI II).
4. Under the guidance of scientific supervisor is able to plan and perform experiment with a use of earlier acquired methods; is able to propose methods for desired analyses and judge their usefullness and limitations for particular investigated plant material ( K_U06 BI I, K_U07BI II).
5. Is able of critical analysis of choosen biological problem using literatute and own research data and formulate his own opinions and
conclusions (K_U08 BI II).
6. Is able to present experimental works in biology field with the use of verbal communication as well as multimedial (K_U09 BI II)
COMMUNITY COMPETENCES
1. Recognizes, what means investigating ethics and reliability in conducting research and interpretation of obtained results (K_K03 BI I, K_K06 BI II).
2. Feels desire of constant upgrading of his education and actualisation of knowledge concerning plant biology, using scientific and popular literature sources K_04 BI I, K_K07 BI II).
3.Understands necessity of upgrading of professional skills (K_K06 BI I).
4. Is capable of working in a team in realisation of his own research and to coordinate work of the whole team ( K_K07 BI I, K_04 BI II).
5. Feels responsible for judgement of safety resulting from applied investigating technics and creation ergonomic and safe conditions of work (K_K05 BI I, K_K08 BI II).
Assessment criteria
Laboratory - multimedial presentation. No more than single absence is allowed during laboratory exercises
Lecture - written exam (open questions, test) in which knowledge presented during the lecture and gained during the laboratory will be evaluate.
Practical placement
none
Bibliography
1.Biotechnologia roślin (red. Stefan Malepszy, Warszawa, PWN 2001),
2.Biotechnologia molekularna (Jerzy Buchowicz, Warszawa PWN 2006),
3.Komórki roślinne w warunkach stresu. Tom II (red. Adam Woźny i Krystyna Przybył, Wydawnictwo Naukowe UAM, Poznań 2004).
4.Plant Tissue Culture Techniques and Experiments ( Roberta H. Smith, Academic Press, ISBN #0-12-650342-7).
5. Laboratory Manual (http://www.biol.uw.edu.pl/ibebr/dydaktyka)
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: