Molecular Genetics 1400-215GEBM

"Molecular Genetics" course.
Biochemical principles of contemporary molecular genetic methods.
This course covers the theory and practice of DNA and RNA manipulation and analysis including:
I. Analysis of the nucleic acids in silico.
II. DNA amplification by PCR and PCR product analysis.
III. Construction of expression cassettes: genomic DNA isolation, PCR, DNA electrophoresis, DNA recombination, E. coli transformation, plasmid DNA isolation, plasmid DNA digestion by restriction endonucleases, restriction map construction, DNA sequencing, site specific mutagenesis.
IV. Construction of S. cerevisiae strains expressing TAP tagged proteins by:
a/ in vitro (PCR) DNA recombination, b/ S. cerevisae transformation and c/ analysis of modified S. cerevisiae strains by PCR and Western blot.V. RNA analysis: RNA isolation, RNA electrophoresis, Northern blot (including molecular probe preparation and labeling).
VI. Quantitative nucleic acids analysis - real-time PCR and real-time RT-PCR.