Cell physiology 1400-215CYTO
1) Regulation of meiotic division in mouse oocytes.
Practical class: Isolation of mouse oocytes in prophase of the 1st meiotic division and their in vitro culture; immunostaining of the metaphase spindle; microscopic analysis of the immunostained cells.
2) Sermatozoon as an example of a motile cell.
Practical class: Isolation of mouse spermatozoa; examination of the effect of cytoskeletal and mitochondrial inhibitors on sperm motility.
3) Regulation of fertilization in mouse.
Computer work: analysis of Ca2+ oscillations induced in oocytes by fertilization.
4) Regulation of mitotic divisions in mouse embryos.
Computer work: morphokinetic analysis of time-lapse movies of dividing embryos.
5) Establishment of cellular junctions and cell polarity in compacting mouse embryos.
Practical class: Isolation of 8-cell embryos, examination of the effect of cytoskeletal and l inhibitors and Ca2+ chelators on compaction of the embryos.
6) Signalling pathways, ligand-receptor interaction; autocrine and paracrine signalling - FGF signalling in mouse embryos.
Computer work: analysis of the effect of FGF signalling modulation on number of cells in the first embryonic cell lineages in mouse blastocysts.
7) Mast cells and their role in homeostasis and immune response.
Computer work: a presentation prepared by students based on the literature provided by the course instructors.
Practical class: isolation of mast cells, assay for a degranulanulation induced by parasite antigens, metachromasia of mast cells.
8) P-glycoprotein pomp (PgP) activity and its role in drug resistance in parasites.
Computer work: a presentation prepared by students based on the literature provided by the course instructors.
Practical class: examination of the effect of antiparasitic agents on a PgP activity - egg hatching assey for Heligmosomoides polygyrus, analysis of the chitosan and saponin effect on the parasite survival.
9) Signal transduction in cells: the influence of ES on the expression of Jak/STAT pathway genes in fibroblasts.
Practical class: determining the effect of the excretory-secretory antigen (ES) of the nematode Trichinella spiralis on the expression of Jak/STAT pathway genes in fibroblasts using droplet digital PCR.
Computer work: determining the role of individual proteins in the Jak/STAT pathways using the Human Protein Atlas and recommended literature.
10) Bacterial infection and a cellular response to it.
11) Cell death - types and regulation.
Examination of the reaction of immune cells to LPS; flow cytometry analysis of activation and apoptosis of the examined cells.
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
KNOWLEDGE
K_W05 The graduate has a thorough knowledge and understanding of the rules and cellular mechanisms of organism development and functioning.
K_W06 The graduate has a thorough knowledge and understanding of specialized concepts in cytophysiological terminology and the literature in these areas.
K_W09 The graduate has a thorough knowledge and understanding of specialized bioinformatics tools necessary for solving problems in the biological sciences studied.
K_W10 The graduate has a thorough knowledge and understanding of various research techniques and tools used in biological sciences (in the field of cytophysiology) and properly plans their use to solve assigned tasks, including those in microbiology in its broadest sense.
K_W13 The graduate has a thorough knowledge and understanding of the principles of research planning and conducting experiments using specialized methods used in cytophysiology. K_W16 The graduate knows and understands occupational health and safety and ergonomics principles that ensure safe laboratory work.
SKILLS
K_U01 The graduate is able to apply research techniques and tools appropriate to the problems of the biological sciences specialization studied.
K_U03 The graduate is able to proficiently use scientific and popular science biological texts in both their native language and English.
K_U08 The graduate is able to critically analyze a selected biological problem based on literature data and their own research results, formulating their own opinions and conclusions.
K_U09 The graduate is able to critically present research papers in the field of the selected biological sciences specialization using verbal communication and multimedia.
SOCIAL COMPETENCES
K_K04 The graduate is ready to work in a team, conducting their own research and co-organizing the work of the entire team.
K_K06 Graduates are ready to apply the principles of research ethics when resolving professional dilemmas. K_K07 Graduates are ready to continuously improve their education and update their knowledge, using scientific and popular science sources related to specialized biological sciences.
Assessment criteria
The final credit for the course is passing a written exam. Admission to the written exam is conditional upon passing the exercises, which are based on attendance (two absences are permitted). The written exam covers information provided by the instructor during the introduction to the course and from recommended reading. The written exam will include multiple-choice and multiple-choice questions and short essay questions.
Practical placement
n/a
Bibliography
1) Podstawy biologii komórki, Bruce Alberts i in., PWN or its English original version
2) Cell Physiology. Source book, ed. N. Sperelakis, Academic Press
3) Podstawy Cytofizjologii, ed. J. Kawiaka, J.Mireckiej, M.Olszewskiej i J. Warchoła, PWN
4) Immunologia, Roitt, Brostoff, Male, PZWL
5) Ćwiczenia z biologii rozwoju zwierząt ed. M. Maleszewskiego. Wydawnictwa UW
6) Developmental Biology, Scott F. Gilbert, Sinauer Associates, Inc.
7) artykuł Maria Doligalska, Kinga Jóźwicka, Mauryla Kiersnowska, Agnieszka Mroczek, Cezary Pączkowski, Wirginia Janiszowska. Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri. VETERINARY PARASITOLOGY Tom 30 Nr 179 r. 2011, str. 144-151
Additional information
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