Cell physiology 1400-215CYTO
1) Regulation of meiotic division in mouse oocytes.
Practical class: Isolation of mouse oocytes in prophase of the 1st meiotic division and their in vitro culture; immunostaining of the metaphase spindle; microscopic analysis of the immunostained cells.
2) Sermatozoon as an example of a motile cell.
Practical class: Isolation of mouse spermatozoa; examination of the effect of cytoskeletal and mitochondrial inhibitors on sperm motility.
3) Regulation of fertilization in mouse.
Computer work: analysis of Ca2+ oscillations induced in oocytes by fertilization.
4) Regulation of mitotic divisions in mouse embryos.
Computer work: morphokinetic analysis of time-lapse movies of dividing embryos.
5) Establishment of cellular junctions and cell polarity in compacting mouse embryos.
Practical class: Isolation of 8-cell embryos, examination of the effect of cytoskeletal and l inhibitors and Ca2+ chelators on compaction of the embryos.
6) Signalling pathways, ligand-receptor interaction; autocrine and paracrine signalling - FGF signalling in mouse embryos.
Computer work: analysis of the effect of FGF signalling modulation on number of cells in the first embryonic cell lineages in mouse blastocysts.
7) Mast cells and their role in homeostasis and immune response.
Computer work: a presentation prepared by students based on the literature provided by the course instructors.
Practical class: isolation of mast cells, assay for a degranulanulation induced by parasite antigens, metachromasia of mast cells.
8) P-glycoprotein pomp (PgP) activity and its role in drug resistance in parasites.
Computer work: a presentation prepared by students based on the literature provided by the course instructors.
Practical class: examination of the effect of antiparasitic agents on a PgP activity - egg hatching assey for Heligmosomoides polygyrus, analysis of the chitosan and saponin effect on the parasite survival.
9) Signal transduction in cells: the influence of ES on the expression of Jak/STAT pathway genes in fibroblasts.
Practical class: determining the effect of the excretory-secretory antigen (ES) of the nematode Trichinella spiralis on the expression of Jak/STAT pathway genes in fibroblasts using droplet digital PCR.
Computer work: determining the role of individual proteins in the Jak/STAT pathways using the Human Protein Atlas and recommended literature.
10) Bacterial infection and a cellular response to it.
11) Cell death - types and regulation.
Examination of the reaction of immune cells to LPS; flow cytometry analysis of activation and apoptosis of the examined cells.
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
The student knows in-depth selected theoretical and practical aspects (including techniques and methods) of cell biology and cytophysiology and is able to use this knowledge, incl. in broadly understood microbiology (S1_W01).
The student applies and disseminates the principles of interpreting phenomena and processes occurring in eukaryotic cells based on empirical data resulting from their experiments (S1_W02).
The student understands the basic (bio)ethical and legal rules related to the use of animal material in scientific and didactic activities (S1_W05).
The student is able to formulate and solve scientific problems in cell biology and cytophysiology by conducting experiments, proper selection of sources and advanced research methods as well as by their critical evaluation in the light of current knowledge and is able to assess their relationship to other biological issues, incl.those of general microbiology (S1_U01).
The student is able to critically evaluate scientific and popular science content (S1_K01).
Assessment criteria
In order to be allowed to take the exam, students must attend the class (up to 2 absences are allowed).
The scope of the exam includes information provided by the instructor during the introduction to the classes, as well as from the literature recommended by the instructor.
The exam is in the form of an open test and may include descriptive questions.
Practical placement
n/a
Bibliography
1) Podstawy biologii komórki, Bruce Alberts i in., PWN or its English original version
2) Cell Physiology. Source book, ed. N. Sperelakis, Academic Press
3) Podstawy Cytofizjologii, ed. J. Kawiaka, J.Mireckiej, M.Olszewskiej i J. Warchoła, PWN
4) Immunologia, Roitt, Brostoff, Male, PZWL
5) Ćwiczenia z biologii rozwoju zwierząt ed. M. Maleszewskiego. Wydawnictwa UW
6) Developmental Biology, Scott F. Gilbert, Sinauer Associates, Inc.
7) artykuł Maria Doligalska, Kinga Jóźwicka, Mauryla Kiersnowska, Agnieszka Mroczek, Cezary Pączkowski, Wirginia Janiszowska. Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri. VETERINARY PARASITOLOGY Tom 30 Nr 179 r. 2011, str. 144-151
Additional information
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