Industrial Microbiology - W 1400-114MIKP-W

1.Introduction (definition and history: from traditional industrial microbiology to microbial biotechnology). This lecture highlights many fascinating aspects of microbial biotechnology. The impact of functional genomics, proteomics, metobolomics on the future prospects of fermentation microbiology are discussed.
2.Industrially important microorganisms i.e. bacteria (actinomycetes), yeasts and molds. Characterization of microorganisms which play a central role in the production of a wide range of primary and secondary metabolites, enzymes, antibiotics and other industrial chemicals. Description of the metabolic routes. Primary and secondary metabolism - current advances and future prospects.
3.Microbial enrichment, isolation, screening and selection of useful microorganisms. Genetic engineering for strain improvement. Applied aspects of genetic engineering and basic biotechnology techniques for the construction of industrial microorganisms and strains producing high-value recombinant products.
4.Characteristics of large scale fermentation. The type of culture, fermentation methods, fermentor design, instrumentation, sterilization, downstream processing and product recovery.
5.Major products of industrial microbiology. Antibiotics, their isolation and characterization - the mode of action, and spectrum of activity. The biosynthetic pathways of natural and semisyntetic compounds belonging to the class of b-lactams, tetracyclines, macrolides, aminoglycosides and polypeptide antibiotics.
6.The phenomenon of feedback mechanism responsible for the regulation of anabolic pathways for the biosynthesis of amino acids. Amino acids used in the medicine, food and chemical industry. Biosynthesis of L-glutamic acid and L-lysine. Genome-based strain reconstruction strategies.
7.Microbial processes developed for the production of food additives such as citric acid, lactic acid and vinegar.
8.Microbial bioconvertion. Principles of the process and applications. Biotransformation of steroides using microorganisms.
9.Types of major industrial enzymes, their modifications and application. Enzymes and their desired modification. Alteration of physical parameters of subtilizine and xylanase. Cell and enzyme immobilization and its application in biotechnology. Current trends and future prospects.