Gas chromatography 1200-SPORSchg
The following issues will be discussed during capillary gas chromatography classes: separation mechanism, retention values, interactions in solution, stationary phases, retention indexes, capillary chromatographic columns, peak resolution, temperature programming, sample dosing in capillary gas chromatography: with and without division, cool on-column injection and temperature programmable injector (PTV). Chromatographic columns: structure of columns; selection of the column for analysis; separation degree: - separation of non-dividing signals; carrier gas flow velocity - Van Deemter curve; the shape of the chromatographic signal; detectors: flame ionization detector, electron capture detector, other detectors; qualitative and quantitative analysis.
The theoretical knowledge obtained during the lecture is supplemented by four classes in the laboratory:
1. Capillary gas chromatography - basics,
2. Capillary gas chromatography; qualitative analysis based on retention indexes,
3. Capillary gas chromatography, liquid phase polarity,
4. Determination of aromatic hydrocarbons in water by extraction method (internal standard).
Type of course
Course coordinators
Learning outcomes
After completing the course, the student will understand the advanced problems of trace analysis of organic compounds in the environment using capillary gas chromatography as an analytical technique.
Assessment criteria
Discussion during the lecture, processing of data obtained during work in the laboratory, final exam.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: