Thymidine/alcohol/surfactant/water system studies with UV-vis spectroscopy 1200-PROJ17
I. Theoretical part covers: UV-vis spectroscopy theoretical background: electromagnetic field interaction with matter; origin of UV-vis spectra, absorbance, molar extinction coefficient, the Beer law, the Beer-Lambert law, qualitative and quantitative UV-vis applications. UV-vis spectrophotometer unit and principle of operation. Thymidine - physicochemical properties, surfactants – types and general characteristic, aggregation ability into micelles and reversed micelles, CMC (critical micelle concentration).
II. Experimental part consists of: getting skills in UV-vis system operating, preparation of surfactant and thymidine solutions, CMC detection for selected systems AOT/solvent system, Thymidine spectra registration in the presence of AOT.
Course coordinators
Learning outcomes
Theoretical and practical learning of UV-vis methods for CMC detection of surfactants in selected solvents.
Assessment criteria
Form of assessment: Report
Bibliography
H.Charlotte Grinter „UV-vis spectroscopy and its applications” Springer Laboratory, 1992
Robert Bauman, “Absorption spectroscopy”, John Wiley & Sons Inc., New York London, 1962.
H.H. Jaffa & Milton Orchin “Theory and application of ultraviolet spectroscopy” John Wiley & Sons Inc., New York London 1962.
Spectrophotometer Cary WinUV Varian manual
Related scientific papers.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: