Trace analysis of the organic pollutants in the environment using capillary gas chromatography 1200-2SPEC392M
1. Capillary gas chromatography: separation mechanism, interaction in solution, stationary phases, retention index, signal separation, capillary columns, temperature programming, detectors, qualitative and quantitative analysis.
2. Gas chromatography-mass spectrometry: mass spectrometry basics, ionization methods, high resolution and quadrupole spectrometers, qualitative analysis using GC/MS, single ion monitoring (SIM), tandem mass spectrometry (MS/MS), liquid chromatography-mass spectrometry (LC/MS), solving a structure using mass spectrometry.
3. Sampling and sample preparation prior to chromatographic analysis: (i) air: whole air sampling, adsorptive sample enrichment, cryogenic sampling, analytes recovery; (ii) water: sampling, liquid-liquid extraction, solid phase extraction (SPE and SPME), head space analysis; (iii) soil: preparation of soil samples, Soxhlet extraction,, sample clean up and enrichment. Uncertainty in environmental analysis.
Estimated student workload per semester: 70 hours, including:
• 30 hours of class participation
• 15 hours of consultations
• 25 hours of exam preparation
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
Upon completion of the course, students will be able to explain the basic principles of trace organic pollutant analysis.
The course aims to provide a solid foundation for anyone pursuing higher education or professional careers in the analysis of trace organic pollutants using capillary gas chromatography, gas chromatography-mass spectrometry (GC/MS), and methods for isolating and concentrating organic pollutants from environmental matrices.
KNOWLEDGE: Upon completion of the course, the student will know and understand:
K_W05 – possesses in-depth knowledge and skills in a selected chemistry specialization, enabling the application of methods and concepts appropriate for this specialization and enabling independent research.
SKILLS: Upon completion of the course, the student will be able to:
K_U03 – apply appropriate research methods, techniques, and tools within a given chemistry specialization, necessary to solve a defined problem.
K¬U08 – Demonstrate advanced knowledge and skills enabling effective use of professional literature, databases, and other sources of information, as well as critically assess the credibility of obtained information.
K_U13 – Independently acquire knowledge and develop professional skills using various sources (written and electronic), including those in foreign languages. SOCIAL COMPETENCES: After completing the course, the student is ready to:
K_K01 – Undertake continuing education activities and independently search for information in literature, including in foreign languages.
Assessment criteria
test
Practical placement
NA
Bibliography
1. Z. Witkiewicz, W. Wardencki Chromatografia gazowa. teoria i praktyka PWN, Warszawa 2018.
2. R. A. W. Johnstone, E. R. Malcolm, Spektrometria mas. Podręcznik dla chemików i biochemików,(Mass spectrometry for chemists and biochemists), PWN, Warszawa, 2001.
3. J. Namieśnik, Z. Jamrógiewicz, M. Pilarczyk, L. Torres, Przygotowanie próbek środowiskowych do analiz (Preparation of environmental samples for analysis), WNT, 1999.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: