Application of Scanning Probe Microscopy to Biological Samples 1200-2RMON2L
Fundamentals of scanning tunneling microscopy (STM) and electrochemical scanning tunneling microscopy (EC-STM). Application of STM and EC-STM techniques for structural studies of biomimetic membranes including lipid films. Application of STM for molecular recognition imaging, identification of functional groups and metallic centers within the proteins, electron transfer through organic molecules.
Fundamentals of atomic force microscopy (AFM). Different AFM modes: Contact (C-AFM), Non-contact/Intermittent contact (NC/IC-AFM), Conductive Probe (CP-AFM), Lateral Force (LFM), Electrostatic Force (EFM), Chemical Force (CFM), Force Spectroscopy. Imaging and probing of biological samples, biomimetic membranes and cells. Measurements of adhesion forces on model membranes. Chemical specificity in AFM-based imaging. Modification of AFM probes with (bio)molecules for molecular recognition imaging and the identification of specific membrane regions.
Type of course
Mode
Course coordinators
Learning outcomes
General knowledge about
• the fundamentals of STM and AFM techniques and the mechanism of contrast
• how to select proper SPM technique for particular sample
• application of STM and AFM beyond conventional imaging – especially for biological and biomimetic samples.
Assessment criteria
Assessment in the form of a quiz. The pass mark is to obtain a minimum of 51% of the maximum number of points in the quiz. The permissible number of excusable absences is 2.
Bibliography
1. "Scanning Probe Microscopies Beyond Imaging Manipulation of Molecules and Nanostructures" ed. Paolo Samori, Wiley VCH, Weinheim 2006.
2. "A practical Guide to Scanning Probe. Microscopy.", R. Howland, L. Benatar. Park Scientific Instruments, 1993-1996
3. P.W. Atkins "Physical Chemistry" Oxford Univeristy Press, 2006.
4. 4. "Review of Progress in Atomic Force Microscopy" S. Maghsoudy-Louyeh, M. Kropf, B. R. Tittmann, 2018, DOI: 10.2174/1874440001812010086.
Additional information
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