Role of Metal Ions in the Structure of Biologically Relevant Compounds 1200-2MON6Z

Detailed analysis of structural and catalytic role of metal ions in proteins, enzymes and DNA. Relation between properties of metal ions and complexing abilities of biomolecules. Biosensors: definition, classification, basic operating principles, history of commercial biosensors with a focus on amperometric biosensors. First, second and third generation of amperometric biosensors: redox reactions, oxidation and reduction of hydrogen peroxide, use of redox active mediators, electroanalytical methods of detection, commercially available glucose biosensors, application of glucose biosensors in food and drink industries, detection of alcohols and aldehydes, detection of toxic metal ions. Biofuel cells: reduction of oxygen, use of laccase and other metal containing enzymes. Interactions of DNA with alkali and transition metal ions. DNA biosensors: principles of operation and applicability, electroanalytical methods of detection, use of complexes of transition metal ions as mediators of the oxidation of DNA intercalators

Type of course

elective monographs

Prerequisites (description)

Basic informations on the relation between the structure of biologically relvant compounds such as peptides, enzymes and DNA and their complexation by elkali earth and transition metal ions. Application of electroanalytical methods in biosensors. Ability to present literature and/or own reseach topics.

Course coordinators

Hanna Majewska-Elżanowska

Learning outcomes

Understanding the relations between metal ion complexation and the properties of the biologically relevant compounds. Basic knowledge of enzymatic biosensors, the ones already produced and the others under development. Understanding of the principles governing the formation of DNA biosensors. Knowledge of electroanalytical methods used for the detection in biosensors.

Assessment criteria

Test and report on chosen topic.

Practical placement

There is no practice available for this subject.

Bibliography

1.S. J. Lippard, J. M. Berg, Podstawy chemii bionieorganicznej, Wydawnictwo Naukowe PWN, Warszawa 1998.
2.J.J.R. Frausto da Silva and R.J.P. Williams, The biological chemistry of the elements, Oxford University Press , Oxford, 2001.
3.W. Schuhmann, Reviews in Molecular Biotechnology, 82 (2002) 425-441.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 1200-2MON6Z in USOSweb