Practical Applications of Raman Spectroscopy 1200-2MON13Z

Short characteristics of Raman spectroscopy techniques: normal Raman scattering, resonance Raman (RR), surface enhanced Raman scattering (SERS), tip enhanced Raman spectroscopy (TERS), coherent anti-Stokes Raman spectroscopy (CARS) and time resolved Raman spectroscopy. Comparison of abilities of imaging techniques: mapping with normal Raman, CARS and TERS Presentation of utility of particular techniques in studies of surface processes, crystalline structure, kinetics of chemical reactions, nanomaterials, polymers, important biological molecules, micro-organisms (bacteria and viruses) and living organisms components (nucleic acids, cells and tissues). Demonstration of applications of Raman spectroscopy techniques in archeology, art, art conservation, medicine (including forensic), pharmacy, geology, astrobiology, and analytical chemistry, including difficulty or quantitative analysis based on Raman spectra/maps. During the course the following issues will be discussed in details: possibility of in vivo Raman measurements, principle of Raman optical tweezers, Raman spectrometer searching for life on Mars, determination of DNA sequence with TERS spectroscopy, in situ SERS detection of water pollution with polycyclic aromatic hydrocarbons in Gulf of Gdańsk, monitoring of drug distribution and its interactions with cells in biological cells via imaging with Raman maps, principle of operation of intracellular pH sensor based on SERS spectroscopy, analysis of pigments used in works of art with RR and SERS techniques, RR spectroscopy as a tool for analysis of car paints in order to apprehend offenders of ‘hit and run’ accidents, disease diagnosis ( with Raman techniques, imaging of cells, tissues and metabolites with CARS spectroscopy, detection and quantitative determination of label-free cocaine with SERS using nanoprobes (carbon nanotubes modified with silver nanoparticles) coupled with antibodies reacting selectively with drug metabolites, recognition of electron transfer mechanism in systems mimicking biological processes with time resolved SERRS spectroscopy, identification of bacteria by means of SERS technique.