Specialization Laboratory 1200-2CHMPRSP1M

The course acquaints the student with the theoretical foundations and practical use of research and analytical techniques as well as elements of organic synthesis that may be useful during the implementation of the diploma thesis. The topics of the specialization exercises are related to the scope of research work carried out in the didactic units of ZChOiTCh and ZChNiA. As part of the specialization lab, students complete 14 exercises out of 16 available. It should be noted that in the case of exercises consisting of 2 parts, both parts must be completed. However, in the final calculation, each part is counted as an independent exercise (one of the 14). During the laboratory, exercises on the following topics are implemented:
- Solid Support Peptide Synthesis (SPPS) - part I and II
- Click chemistry: synthesis of a fluorescently labeled compound and its properties - part I nad II;
- Nucleotide derivatives of the 5' end of mRNA - a novel approach to synthesis, purification and identification -part I and II;
- Synthesis and physicochemical characterization of drug delivery systems based on surface-modified gold nanoparticles;
- Analysis of residual solvents in drug active ingredients by capillary chromatography;
- Western blot - part I and II;
- Microscopic imaging of soft structures,
- Use of infrared spectroscopy for structural studies of model cell membranes,
- In search for new pyrazinamide cocrystals;
- Iron oxide nanoparticles - synthesis, characterization and application in magnetic hyperthermia;
- Methodology for preparing biological and/or clinical material for analysis and studying elemental composition by inductively coupled plasma ionization mass spectrometry (ICP-MS) technique;
- Stability analysis of active substances in drugs / dietary supplements using liquid chromatography.