Specialization Laboratory 1200-2CHMPRSP1M
The course acquaints the student with the theoretical foundations and practical use of research and analytical techniques as well as elements of organic synthesis that may be useful during the implementation of the diploma thesis. The topics of the specialization exercises are related to the scope of research work carried out in the didactic units of ZChOiTCh and ZChNiA. As part of the specialization lab, students complete 14 exercises out of 17 available. It should be noted that in the case of exercises consisting of 2 parts, both parts must be completed. However, in the final calculation, each part is counted as an independent exercise (one of the 14). During the laboratory, exercises on the following topics are implemented:
- Solid Support Peptide Synthesis (SPPS) - part I and II;
- Nucleotide derivatives of the 5' end of mRNA - a novel approach to synthesis, purification and identification -part I and II;
- Synthesis and physicochemical characterization of drug delivery systems based on surface-modified gold nanoparticles;
- Western blot;
- Microscopic imaging of soft structures;
- In search for new pyrazinamide cocrystals;
- Iron oxide nanoparticles - synthesis, characterization and application in magnetic hyperthermia;
- Methodology for preparing biological and/or clinical material for analysis and studying elemental composition by inductively coupled plasma ionization mass spectrometry (ICP-MS) technique;
- Stability analysis of active substances in drugs / dietary supplements using liquid chromatography;
- Testing glucose level in blood and sweat;
- FLASH column chromatography and its use in the purification of biologically important compounds;
- Use of the medical analyzer Indiko Plus for determining Ca and Mg in dietary supplements;
- Transport of anions through the lipid bilayer of liposomes using synthetic anionophores;
- Analysis of salt complexation and extraction using supramolecular receptors (UV-Vis studies, fluorimetry);
- Analysis of residual solvents in drug active ingredients by capillary chromatography.
Total student workload per semester - 250 hours, including:
- participation in classes: 120 hours
- preparation of reports: 20 hours
- preparation for classes: 90 hours
- consultations with lecturers: 20 hours
Information regarding individual exercises can be found on the Kampus platform.
Main fields of studies for MISMaP
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
The student knows and understands: K_W02 (aspects of the construction and operation of modern measuring equipment supporting scientific research in a chemical laboratory); K_W15 (principles of ergonomics, occupational health and safety in the laboratory); K_W16 (selected advanced chemical, physical, and biological phenomena and processes).
The student is able to: K_U02 (apply appropriate methods, techniques, research and IT tools necessary to explain the research problem); K_U03 (work in a team (including interdisciplinary teams) with an awareness of responsibility for jointly implemented tasks related to teamwork); K_U12 (independently acquire knowledge and develop their professional skills using various sources, including those in a foreign language); K_U15 (plan and perform experimental research or observations and analyze their results); K_U16 (measure selected physicochemical quantities, determine their values and measurement errors, and assess the reliability of the results obtained); K_U17 (design, assemble, and use selected measuring equipment and apply various measuring systems); K_U18 (plan and define a research objective, plan and carry out its implementation, as well as collect and interpret empirical data).
The student is ready to: K_K01 (continuously improve their skills and independently search for information in literature, including foreign-language sources); K_K02 (comply with professional ethics and require others to do the same (with a conviction of the importance of professional behavior)); K_K04 (critically evaluate scientific and popular science content); K_K07 (critically evaluate the level of their knowledge (in case of difficulties with solving a problem independently, seek the opinion of experts) and independently undertake and initiate simple research activities).
Assessment criteria
The basis for passing the classes of the Laboratory is an execution of 14 exercises, grading each entry / exit tests and grading each report. Each student is entitled to one absence, which must be made up in a make-up lab.
The final grade from the studio results from the weighted average (WA) which is the sum of the arithmetic mean of all entry / exit tests (AmT) with a weight of 0.7 and the arithmetic mean of all reports (AmR) with a weight of 0.3, according to the following formula: WA = 0.7 × AmT + 0.3 × AmR
Practical placement
Not applicable.
Bibliography
Literature appropriate to the topic of the exercise, given by the teacher.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: