Biosensors 1200-2CHMMO2W1
Introduction (definitions, history of development, classification). General principles and schemes of operation, analytical characteristics. Enzymes, immobilizing enzymes and other biomolecules. Monoenzymatic biosensors (electrochemical and optical). Polyenzyme biosensors. Tissue, cellular and bacterial biosensors. Inhibitor biosensors. Affinity biosensors (non-enzymatic bioreceptors). Immuno-diagnosis and gene diagnosis. Immuno and genosensors (with and without markers). DNA / RNA biosensors, hybridization process, methods of introducing DNA / RNA fragments on a selected surface. Ways of visualizing the hybridization process. Labeling of DNA / RNA strands. More important analytical applications of biosensors.
Total student workload: 3x30=90 hours, including:
class participation: 30 hours
class preparation: 40 hours
consultations with instructors: 10 hours
tests: 5 hours
exam: 5 hours
Main fields of studies for MISMaP
Type of course
Mode
Prerequisites (description)
Course coordinators
Learning outcomes
The student knows and understands: K_W07 (principles of operation, properties, and applications of various types of biosensors); K_W10 (design and applications of various types of analytical microchips); K_W11 (current trends in the development of modern medical analytics); K_W16 (selected advanced chemical, physical, and biological phenomena and processes).
The student is able to: K_U08 (design a measurement system for flow analysis with various detection methods); K_U09 (assess the possibilities and limitations of applying various concepts for improving analytical measurements); K_U10 (prepare a validation protocol for a given measurement procedure, and as part of the validation procedure, develop a method for ensuring measurement consistency and propose a method for determining the uncertainty of results).
The student is ready to: K_K02 (comply with professional ethics and require others to do so, convinced of the importance of professional behavior); K_K04 (critically evaluate scientific and popular science content); K_K07 (critically evaluate the level of their knowledge (in case of difficulties with solving a problem on their own, seek the opinion of experts) and independently undertake and initiate simple research activities).
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After completing this course, the student should demonstrate knowledge and understanding of the principles of operation, properties and applications of various types of biosensors.
Assessment criteria
Oral or written exam (two tests).
1. The problem of biosensor and its structure.
2. Methods of creating an analytically active layer / introducing the receptor on a selected surface.
3. Hybridization process and factors influencing it.
4. Structure, division and action of enzymes.
5. Antibodies: structure, division and functions.
6. Techniques of visualization of analyte recognition by the receptor.
Practical placement
Not applicable.
Bibliography
Materials provided by the teacher in the form of scientific publications.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: