Liquid Chromatography 1200-2BLOK4W2

The lecture comprises the following topics:
·retention parameters: flow rate, linear velocity
·retention factor, selectivity factor, resolution R, efficiency N, Purnell’s equition
·classification of chromatographic methods,
·chromatographic theory, parameters describing peak shape, asymmetry factor
·stationary phases in HPLC classification and application
·column selection and instrumentation,
·optimization of experimental conditions,
·application of HPLC for determination different classes of compounds,
·quantitative determination as well as
·perspective of chromatographic methods development

Main fields of studies for MISMaP

chemistry

Type of course

obligatory courses

Prerequisites (description)

It is assumed that a student has knowledge of analytical chemistry chemistry at the level of Basic chemistry, Analytical chemistry and Physical Chemistry taught for of bachelor studies in chemistry at the Faculty of Chemistry, University of Warsaw.

Course coordinators

Ewa Poboży

Learning outcomes

Student knows the basic terms of HPLC and how to use them in practical aspects. Student knows how to calculate the basic separation parameters. Student knows how to develop method of separation for basic compounds according to theirs chemical properties

Assessment criteria

Final examination carried out in a written form. Open quections, time of exam 60 min.
The condition of passing the exam is to obtain over 50% of points.

Practical placement

Bibliography

1.C.Poole, S.K.Poole, Chromatography today, Elservier
2.L.R.Snyder, J.J.Kirkland, J.L.Glajch, Practical HPLC development, Wiley

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 1200-2BLOK4W2 in USOSweb