Liquid Chromatography 1200-2BLOK3-WW2
The lecture comprises the following topics:
·Retention parameters: flow rate, linear velocity
·Retention factor, selectivity factor, resolution R, efficiency N, Purnell’s equition
·Classification of chromatographic methods,
·Chromatographic theory, parameters describing peak shape, asymmetry factor
·Stationary phases in HPLC classification and application
·Column selection and instrumentation,
·Optimization of experimental conditions,
·Application of HPLC for determination different classes of compounds,
·Quantitative determination
Main fields of studies for MISMaP
Type of course
Mode
Course coordinators
Learning outcomes
The graduate knows and understands:
(K_W04) aspects of the construction and operation of modern measuring equipment supporting scientific research in chemistry.
(K_W05) Possesses in-depth knowledge and skills in the chosen chemical specialization, enabling the use of methods and concepts appropriate for this specialization and enabling independent research.
(K_W07) Knows and understands, and can independently explain, the mathematical description of basic chemical phenomena and processes.
(K_W10) Has a good understanding of current developments in chemistry and the latest scientific discoveries in a given chemical specialization.
The graduate is able to:
(K_U03) Apply appropriate methods, techniques, and research tools within a given chemical specialization, necessary to explain the problem.
(K_U05) Possesses the ability to independently plan and conduct theoretical research within their chemical specialization. (K_U13) Is able to independently acquire knowledge and develop professional skills using various sources (written and electronic), including those in foreign languages.
Social competences:
(K_K01) Understands the need for and is aware of the opportunities for continuous learning. Is able to independently search for information in literature (including foreign languages) and seek expert advice.
(K_K06) Is able to think and act in a creative and entrepreneurial manner.
Assessment criteria
Final examination carried out in a written form. Open quections, time of exam 60 min.
The condition of passing the exam is to obtain over 51% of points.
Practical placement
No
Bibliography
1.C.Poole, S.K.Poole, Chromatography today, Elservier
2.L.R.Snyder, J.J.Kirkland, J.L.Glajch, Practical HPLC development, Wiley
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: