Bioanalytics of Natural Samples 1200-2BLOK3-AW3

Tasks to be realised include: defining the research objective and planning its implementation; collecting biological and environmental samples to ensure sample stability; methods for sample preservation to maintain primary stability; identifying sources of errors in sample analysis while maintaining representativeness; relationship between the form of element occurrence and its toxicity; sources of errors in speciation analysis; analytical methods used for determining different analyte forms; methods of analyte extraction from sample matrices (mineralisation, solvent and assisted extractions, solid-phase extractions); definition and construction of sensors/biosensors, with special emphasis on sensors with electrochemical detection; methods receptor layers formation and effective immobilisation of the bio-receptors to maintain their activity towards the analyte; Impact of receptor packing density, orientation of the immobilised receptor, and receptor layer regularity on effectiveness of the analyte recognition process; planning sensor construction for a selected detection method; examples of sensor/biosensor applications; introduction to fundamental norms (ISO) concerning chemical analysis, with a particular focus on elemental and molecular analysis; discussion on the validation stage of analytical methods, validation criteria, and their importance for ensuring result quality; the significance of reference materials in the process of elemental and molecular analysis, preparation, storage, and use procedures for certified reference materials (CRM); practical examples of analysis applications in environmental and clinical contexts, illustrating the importance of adhering to norms and regulations; review of commonly used analytical techniques in elemental analysis (e.g., atomic absorption spectrometry) and molecular analysis (e.g., mass spectrometry) - advantages and limitations.