Biochemistry and Molecular Biology Laboratory B 1200-1CHMLBIOB4
Students will perform exercises including isolation, purification and quantitative determination of proteins, determination of enzymatic activity and determination of kinetic parameters of selected enzymes, preparation of competent cells, transformation of plasmid DNA, its isolation, preparative PCR, preparation of DNA for sequencing and ligation of DNA into a plasmid vector. The course includes 8 exercises.
During the proseminar classes, methods of working with proteins and enzymes (isolation, purification), methods of their quatitative determination, enzymes used in molecular biology and ideological foundations (with particular emphasis on chemical aspects) of basic methods of molecular biology (including sequencing, PCR, electrophoresis )will be discussed
Type of course
Mode
Requirements
Prerequisites (description)
Course coordinators
Learning outcomes
The student knows and understands:
K_W09 : at an advances level chemical components of living organisms, their structural features, chemical properties and role in biological processes, knows and understands the relationship between the structure of biomolecules and their function.
K_W18 : at an advances level aspects of the construction and operation of modern measuring apparatus supporting scientific research in chemistry, biochemistry and molecular biology.
K_W19 : at an advances levelsafety rules sufficient to work in a chemical, biochemical and molecular biology laboratory
The student is able to:
K_U10 : identify selected organic and bioorganic compounds using chemical, biochemical and molecular biology, chromatographic and spectroscopic biology techniques
K_U15: plan and perform basic research, experiments, observations, and computer simulations in the fields of chemistry, biochemistry, and molecular biology, as well as critically evaluate their own results and discuss measurement errors.
K_U16: design, assemble, and use selected measuring equipment, conduct measurements of selected physicochemical quantities, determine their values, and assess the reliability of the obtained results.
Assessment criteria
Class attendance (laboratories and proseminars) is compulsory. The student may have a maximum of two overdue exercises resulting from excused absence or non-admission to perform the exercise. Catching up overdue exercises takes place on dates agreed with instructors. Additional classes are also included in the schedule.
Assessment and credit methods:
1)Laboratory: each exercise - 10 points (admission test- 5 points and report - 5 points). You can get 80 points in total.
2) Final test 50 points. To pass the test, it is necessary to obtain 60% of the points. It is also a condition for passing the classes. In case of obtaining a smaller number of points, one should take a retake test in the session. Failure to obtain a minimum of 60% of points will result in failure of the Laboratory in biochemistry and molecular biology.
3) A total of 130 points can be obtained from the laboratory exercises and the test.
To pass the course, you must obtain a minimum of 78 points.
Total student workload - 180 hours, including:
class participation - 90 hours,
class preparation - 20 hours,
report preparation - 35 hours,
final exam preparation - 20 hours,
consultations with instructors - 15 hours
Bibliography
"Ćwiczenia z biochemii" L. Kłyszejko-Stefanowicz and other sources recommended by instructors.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: