Medical Biochemistry 1200-1CHMBMED4
Catalytic proteins - enzymes – introduction, enzymatic reactions and their mechanisms, enzyme kinetics (active site, enzyme-substrate complex, Michaelis-Menten kinetics, Lineweaver-Burk plot, Km, types of inhibitor (competitive, non-competitive, mixed), allosteric enzymes – enzymatic measurement of blood glucose (endpoint versus kinetic assays), regulation of enzymatic activity. Signal transduction pathways, hormones, plasma memrane receptors: G-protein coupled receptors, tyrosine kinase roceptors. Bioenergetics and oxidative metabolism – biologic oxidation and oxidative phosphorylation. Metabolism of carbohydrates, glyconeogenesis, gluconeogenesis, tricarboxylic acid cycle and the pentose phosphate pathway. Biosynthesis and storage of fatty acids, ketogenesis, biosynthesis of cholesterol and steroids, lipoproteins, diabetes mellitus, NASH, familial hypercholesterolemia etc. Biosynthesis and degradation of amino acids, metabolism of dietary and endogenous proteins, amino acid degradation, diseases of amino acid metabolism. Heme synthesis and iron metabolism – metabolic diseases. Nucleotides metabolism. Cellular homeostasis, cell cycle regulation, apoptosis and cancer.
Type of course
Mode
Requirements
Prerequisites (description)
Course coordinators
Learning outcomes
The student knows the basic chemical components of living organisms, their structural features, chemical properties and role in biological processes, knows and understands the relationship between the structure of biomolecules and their function.
The student knows and understands metabolic processes and their chemical mechanisms occurring in the human body, how they are regulated and the effect of toxic substances on their operation.
The student has basic biological knowledge in the field of physiological processes and the functioning of organs of the human body and the medical effects of disorders of metabolic processes occurring there. He knows the structure of the cell and the role and operation of the basic structures present in it.
Assessment criteria
Lectures and seminars are obligatory. Acceptable limit of absence is 3 lectures and 1 seminar.
During the seminars, there will be 4 short tests on the material covering the lecture and the seminars. In order to be admitted to the examination, one should gain a minimum 60% of points. In case of receiving a lower number of points, it is necessary to take a written test covering the whole material and obtain a positive mark (min. 60% of the points). In the case of receiving a lower number of points, it is necessary to take a revision test in the revision session. In the case of an unsatisfactory grade from the re-sit examination, the student is not allowed to take the examination.
The final examination in the subject in written form. To obtain a positive mark it is necessary to obtain min. 60% of the points.
Bibliography
1. Biochemistry (Lippincott Illustrated Reviews) Denise S. Ferrier
2. Biochemistry Berg Jeremy M., Stryer Lubert, Tymoczko John L., Gatto Gregory
3. Essential Cell Biology Bruce Alberts, Karen Hopkin, Alexander Johnson, Martin Raff, Keith Roberts, Peter Walter
Additional information
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