Preclinical Trials of Radiopharmaceuticals - Laboratory 1200-1CHJBPRL5
The lab's exercises will cover the following topics:
- radionuclide production (reactor production, cyclotron production, radionuclide generators, and supply of radionuclides to the Polish market)
- operation of 68Ge/68Ga radionuclide generators,
- synthesis of gallium-labeled radiopharmaceuticals,
- separation methods,
- activity measurements of radioactive sources (operation of ionizing radiation counters and detectors, gamma spectrometry, alpha spectrometry),
- quality analysis,
- cell passaging,
- cell counting and preparation for plating,
- cytotoxicity assay,
- receptor affinity assessment,
- internalization assessment.
Type of course
Mode
Course coordinators
Term 2025Z: | Term 2024Z: |
Learning outcomes
Upon completion of this course, students should demonstrate knowledge of:
- principles of safe work with radioactive isotopes, open and sealed radioactive sources,
- designing radionuclide production,
- operating radionuclide generators,
- operating ionizing radiation detectors,
- interpreting ionizing radiation spectra,
- separation methods,
- methods of cell culture and the importance of aseptic conditions in laboratory work,
- methods for assessing cytotoxicity, internalization, and receptor affinity used in preclinical studies of radiopharmaceuticals.
Assessment criteria
Grading based on the grade obtained from the entrance exam, laboratory work and the report on the exercise results.
Practical placement
Not applicable.
Bibliography
1. Radiopharmaceutical Chemistry. Redaktorzy: Albert D. Windhorst, Brian M. Zeglis, Jason S. Lewis (2019). Niemcy: Springer International Publishing.
2. Choppin, G., Liljenzin, J., Rydberg, J., Ekberg, C. (2013). Radiochemistry and Nuclear Chemistry. Holandia: Academic Press.
3. Gupta, T. K. (2013). Radiation, Ionization, and Detection in Nuclear Medicine. Niemcy: Springer Berlin Heidelberg.
4. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 6th ed. Hoboken: Wiley-Blackwell; 2010. ISBN: 9780470528129.
5. Riss TL, Moravec RA, Niles AL, et al. Cell Viability Assays. In: Sittampalam GS, Coussens NP, Brimacombe K, et al., editors. Assay Guidance Manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2013 May 1 [updated 2016 Jul 1]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK144065/
6. Stokłosowa S, red. Hodowla komórek i tkanek. Warszawa: PZWL; 2011. ISBN: 9788301142322.
7. Benfante V, Stefano A, Ali M, Laudicella R, Arancio W, Cucchiara A, et al. An overview of in vitro assays of 64Cu-, 68Ga-, 125I-, and 99mTc-labelled radiopharmaceuticals using radiometric counters in the era of radiotheranostics. Diagnostics. 2023;13(7):1210. https://doi.org/10.3390/diagnostics13071210
8. Barnard PJ, Bayly SR, Holland JP, Dilworth JR, Waghorn PA. In vitro assays for assessing the potential for copper complexes to function as radiopharmaceutical agents. Dalton Trans. 2008;(30):3927–3933.
9. M. I. Tsionou et al. Comparison of macrocyclic and acyclic chelators for gallium-68 radiolabelling. RSC Adv.,( 2017) 7, 49586–49599.
10. B. J. B. Nelson et al. Good practices for 68Ga radiopharmaceutical production. EJNMMI Radiopharm. Chem. (2022) 7, 27.
11. A. Majkowska, A.Bilewicz, Makrocykliczne kompleksy radionuklidów w medycynie nuklearnej, Wiadomości chemiczne (2008) 62, 7-8
12. https://www.sciencedirect.com/topics/nursing-and-health-professions/radionuclide-generator
13. https://www.polatom.pl/
14. https://www.ncbj.gov.pl/
15. http://www.ichtj.waw.pl/drupal/
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: