Optical microscopy 1100-OM
1. Microscopy contrasts, applications, and challenges; layout of a microscope.
2. Basic concepts, selected types of optical microscopes.
3. Monochromatic field propagation, diffraction.
4. Field propagation through a lens, imaging.
5. Interference-based imaging techniques: optical coherence tomography, holographic microscopy.
6. Fluorescence.
7. Intensity and coherence propagation, light detection.
8. Three-dimensional imaging.
9. Confocal microscopy.
10. Two-photon microscopy.
11. Coherent nonlinear microscopy.
12. Structured illumination microscopy.
13. Super-resolution microscopy: stimulated emission depletion.
14. Super-resolution microscopy: localization.
15. Summary: How to build a microscope.
Main fields of studies for MISMaP
Course coordinators
Mode
General: Remote learning Blended learning | Term 2024L: Classroom |
Learning outcomes
The student will understand the physical foundations and the main techniques of optical microscopy. She/he will be able to identify the limitations and the essential technical aspects of the main optical microscopy techniques.
Assessment criteria
An oral exam covering the material discussed during the lectures.
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: